Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis through Nrf2/NF-κB/NLRP3 axis

Mingxuan Cheng¹, Pu Wang², Di Wu³

¹Department of Prosthodontics, Peking University Hospital of Stomatology First Clinical Division, Beijing 100034; ²School of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081; ³Department of Stomatology, Lishui Peopleâ€™s Hospital (The 6th Affiliated Hospital of Wenzhou Medical University), Lishui City, Zhejiang Province 323020, China.

For correspondence:- Di Wu Email: di_w10@163.com

Accepted: 26 November 2022 Published: 29 December 2022

Citation: Cheng M, Wang P, Wu D. Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis through Nrf2/NF-κB/NLRP3 axis. Trop J Pharm Res 2022; 21(12):2519-2524 doi: 10.4314/tjpr.v21i12.3

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the therapeutic role of diosmetin in periodontitis and its probable mechanism of action.

Methods: Lipopolysaccharide (LPS) was used to induce periodontitis in periodontal cells. Cell viability and apoptosis in response to LPS and diosmetin were evaluated using MTT and TUNEL assays. The oxidative stress and inflammatory responses in LPS-induced periodontitis and diosmetin effects in periodontal cells were detected using enzyme-linked immunosorbent assay (ELISA). In addition, the roles of diosmetin in pyroptosis and Nrf2/NF-kappa B/NLRP3 pathway were analyzed by immunoblot assays.

Results: Diosmetin increased the viability of LPS-induced periodontal cells (p < 0.01). Diosmetin also alleviated the oxidative stress of periodontal cells (p < 0.01), reduced the secretion of pro-inflammatory factors in periodontal cells, and inhibited cell pyroptosis (p < 0.01). Diosmetin also mediated Nrf2/NF-kappa B/NLRP3 pathway (p < 0.01).

Conclusion: Diosmetin alleviates periodontitis by inhibiting oxidative stress and pyroptosis through Nrf2/NF-κB/NLRP3 axis. However, in vivo studies are required to validate this finding

Keywords: Periodontitis, Diosmetin, Oxidative stress, Pyrosis, Nrf2/NF-kappa B/NLRP3 pathway