Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Perfluorocarbon restrains inflammation and cell apoptosis in rats with lung ischemia-reperfusion injury via down-regulation of TLR4/NF-κB signaling pathway

Li Liu, Dan Gou, Yanyan Song, Mingliang Li, Jiwei Gu, Yujing Zhang, Chengliang Qu, Yun Wang* Qu, Yun Wang

Cardiac Macrovascular Surgery, General Hospital of Ningxia Medical University, Yinchuan, China;

For correspondence:- Yun Wang Email: 2386338303@qq.com

Accepted: 28 November 2022 Published: 29 December 2022

Citation: Liu L, Gou D, Song Y, Li M, Gu J, Zhang Y, et al. Perfluorocarbon restrains inflammation and cell apoptosis in rats with lung ischemia-reperfusion injury via down-regulation of TLR4/NF-κB signaling pathway. Trop J Pharm Res 2022; 21(12):2533-2539 doi: 10.4314/tjpr.v21i12.5

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Abstract

Purpose: To exaluate the effect of perfluorocarbon on lung ischemia-reperfusion injury in rats, and to unravel the potential underlying mechanism.

Methods: A total of 36 Sprague-Dawley (SD) rats were randomly assigned to sham group, model group, and perfluorocarbon group (12 rats per group). The levels of inflammatory factors (TNF-α and IL-1β) were determined using enzyme-linked immunosorbent assay (ELISA). Cell apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, while Western blotting was conducted to determine the protein expressions of TLR4 and NF-κB.

Results: The levels of inflammatory factors in the model and perfluorocarbon groups were significantly higher than those in operation group (p < 0.05), while their levels in perfluorocarbon group were significantly lower than in model group (p < 0.05). The mRNA expression levels of TNF-α and IL-1β in lung tissues rose significantly in both model and perfluorocarbon groups when compared with those in sham group (p < 0.05), but declined significantly in the perfluorocarbon group in comparison with those in model group (p < 0.05). Furthermore, the perfluorocarbon group exhibited a significantly lower cell apoptosis than model group (p < 0.05). The relative protein expression levels of TLR4 and NF-κB declined significantly in perfluorocarbon group than in model group.

Conclusions: Perfluorocarbon down-regulates the TLR4/NF-κB signaling pathway, and inhibits inflammation and cell apoptosis after lung ischemia-reperfusion injury in rats, thereby improving their lung function.