Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anisodamine combined with lidocaine improves healing of myocardial ischemia-reperfusion injury in rats via PI3K/Akt signaling pathway

Shouyi Wang¹, Jiahao Chen², Jie Wang³, Hongbo Que³, Daming Wei³, Yanhao Zhu¹, Qin Liu¹, Yu Liu¹, Chunyan Zhu⁴

¹Department of Anesthesiology, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei; ²Department of Cardiology, Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi; ³Department of Anesthesiology, Anhui Integrated Traditional Chinese and Western Medicine Hospital, Hefei; ⁴Department of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing, The Affiliated Hospital of Jiaxing University, Jiaxing, China.

For correspondence:- Chunyan Zhu Email: zhuchunyan308@163.com

Accepted: 11 November 2022 Published: 29 December 2022

Citation: Wang S, Chen J, Wang J, Que H, Wei D, Zhu Y, et al. Anisodamine combined with lidocaine improves healing of myocardial ischemia-reperfusion injury in rats via PI3K/Akt signaling pathway. Trop J Pharm Res 2022; 21(12):2561-2567 doi: 10.4314/tjpr.v21i12.9

© 2022 The authors.
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Abstract

Purpose: To study the effects of anisodamine (Ad) combined with lidocaine (Ldc) on myocardial ischemia-reperfusion injury (MIRI) in rats, and its correlation with PI3K/AKT signaling pathway.

Methods: A total of 70 healthy rats were randomly divided into S group, M group, Ad group, Ldc group, Ad + Ldc group, Ad + Ldc + LY group, and LY group. The cardiac hemodynamic indices in each group were determined, and the area of myocardial infarction measured. Serum biochemical indices were also determined. Furthermore, the protein expressions of p-Akt, T-Akt, Bcl-2, and Bax in myocardial cells were determined by Western blotting.

Results: Compared with those in M group, Ad group, Ldc group, Ad + Ldc + LY group, and LY group, cardiac hemodynamic indices significantly improved, while the area of myocardial infarction was significantly reduced (p < 0.01). Furthermore, serum malondialdehyde (MDA) concentration but the activities of CK, CK-MB, TNF-α, and IL-6 declined, while the activities of superoxide dismutase (SOD), CAT and GSH-Px rose in Ad + Ldc group (p < 0.01). In Ad + Ldc group, p-Akt, T-Akt, and Bcl-2 increased, while Bax significantly decreased. Through comparison LY294002 significantly inhibited the protective effect of Ad combined with Ldc against MIRI in rats (p < 0.01).

Conclusion: Anisodamine combination with lidocaine has a protective effect against MIRI in rats via PI3K/Akt signaling pathway, thus indicating that it is a potential therapeutic strategy for the management of myocardial ischemia-reperfusion.

Keywords: PI3K/Akt pathway, Anisodamine, Lidocaine, Myocardial ischemia-reperfusion