Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Curcumin inhibits expression of brain-derived neurotrophic factor (BDNF) in hippocampus of mice with sleep deprivation

Sha-sha Ruan¹ , Pei-cheng He², Qin-qin Cao¹, Tao Ma¹

¹Department of Neurology, the Affiliated WuXi No. 2 Peopleâ€™s Hospital of Nanjing Medical University, Wuxi, Jiangsu 214000, China; ²Department of medicalâ€‚Imaging, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.

For correspondence:- Sha-sha Ruan Email: ruanlvfdfwv715@163.com

Accepted: 19 December 2022 Published: 30 January 2023

Citation: Ruan S, He P, Cao Q, Ma T. Curcumin inhibits expression of brain-derived neurotrophic factor (BDNF) in hippocampus of mice with sleep deprivation. Trop J Pharm Res 2023; 22(1):99-103 doi: 10.4314/tjpr.v22i1.14

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the inhibitory effect of curcumin on the expression of brain-derived neurotrophic factor (BDNF) in hippocampus of mice with sleep deprivation.

Methods: expressions of BDNF and miRNA-206 were evaluated using quantitative real time-polymerase chain reaction (qRT-PCR) in a sleep-deprived mouse model. Interaction of BDNF and miRNA-206 was assessed by dual luciferase and qPCR) assays. The effect of curcumin on BDNF and miRNA-206 was also determined by qRT-PCR.

Results: expressions of BDNF and miRNA-206 were significantly suppressed in hippocampus of mice in sleep deprivation group compared with control mice (p < 0.05). Treatment with miRNA-206 significantly suppressed the luciferase activity of wild type (WT) BDNF reporter gene vector (p < 0.05) rather than the mutated (mut) vectors. Inhibition of miRNA-206 upregulated RNA and protein levels of BDNF (p < 0.05), while curcumin treatment inhibited BDNF expression (p < 0.01) and upregulated miRNA-206 level (p < 0.01).

Conclusion: Curcumin inhibits BDNF expression by upregulating miRNA-206 level in hippocampus tissues of mice in the sleep deprivation model. The relationship between BDNF and miRNA-206 might provide a new direction for the development of new therapeutics for sleep deprivation.

Keywords: Curcumin, Brain-derived neurotrophic factor, microRNA 206, Sleep deprivation