Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Salvianolic acid A exerts a protective effect against sodium laurate-induced thromboangiitis obliterans in rats

Chaowen Yu, Wenbo Tang, Yuan Tao, Tiancai Ren, Qiwei Chen, Yong Gao

Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, China;

For correspondence:- Yong Gao Email: 18905520887@163.com

Accepted: 19 December 2022 Published: 31 January 2023

Citation: Yu C, Tang W, Tao Y, Ren T, Chen Q, Gao Y. Salvianolic acid A exerts a protective effect against sodium laurate-induced thromboangiitis obliterans in rats. Trop J Pharm Res 2023; 22(1):135-141 doi: 10.4314/tjpr.v22i1.19

© 2023 The authors.
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Abstract

Purpose: To study the potential therapeutic effects of salvianolic acid A (Sal A) on thromboangiitis obliterans (TAO).

Methods: An in vitro model of TAO-mimicking endothelial cell damage was established by incubating ECV304 cells with H₂O₂. An in vivo model of TAO rats was developed via injection of sodium laurate. After treatment with varying doses of Sal A, TAO symptoms were monitored over time and compared. The effects of Sal A on oxidative stress, pyroptosis, inflammation, and thrombosis were assessed using biochemical assays, enzyme-linked immunosorbent assay (ELISA), western blot, and histopathology.

Results: Sal A significantly alleviated the inhibition of cell viability induced by H₂O₂ (p < 0.05). The H₂O₂-induced increases in levels of ROS, IL-1β, and IL-18 in ECV304 cells were significantly decreased by Sal A (p < 0.05). Moreover, Sal A alleviated TAO symptoms in rats. The enhanced levels of IL-1β, IL-18, NLRP3, and active Caspase-1 observed in TAO rats were reduced by Sal A in vivo (p < 0.05). Moreover, Sal A significantly reduced the size of thrombus in TAO rats. In addition, upregulated levels of ROS were significantly inhibited by Sal A (p < 0.05).

Conclusion: Sal A exerts significant therapeutic effects on TAO. This provides mechanistic insights into the clinical effects of RSMA on TAO patients, which might be beneficial in the development of effective drugs against TAO in the future.

Keywords: Inflammation, Salvianolic acid A, Thromboangiitis obliterans, Thrombosis