Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Vitis vinifera seed extract ameliorates streptozotocin-induced pancreatic dysfunction by reducing interleukin-1Î² and up-regulation of omentin-1 mRNA

Karima Bel Hadj Salah^1,2, Diana AH Al-Quwaie¹, Najat Binothman³, Majidah Aljadani³, Manal MS Mansoury⁴, Nouf H Alsubhi¹, Ghadeer I Alrefaei⁵, Ammar A Bayoumi⁶, Maha Ibrahim⁶, Hala A Khattab⁷

¹Department of Biological Sciences, College of Science and Arts, Rabigh Campus, King Abdulaziz University, Jeddah, Saudi Arabia; ²Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99ES27), FacultÃ© de Pharmacie, UniversitÃ© de Monastir, Tunisia; ³Department of Chemistry, College of Sciences and Arts, King Abdulaziz University, Rabigh; ⁴Department of Food and Nutrition, Faculty of Human Sciences and Design, King Abdulaziz University, Jeddah, Saudi Arabia; ⁵Department of Biological Science, Faculty of Sciences, Jeddah University, Jeddah, Saudi Arabia; ⁶Department of Hematology, Medical Research, King Abdulaziz University, Saudi Arabia; ⁷Department of Nutrition and Food Science, Faculty of Home Economics, Helwan University, Egypt.

For correspondence:- Hala Khattab Email: haya_khattab@hotmail.com Tel:+00201005248923

Accepted: 28 January 2023 Published: 27 February 2023

Citation: Salah KB, Al-Quwaie DA, Binothman N, Aljadani M, Mansoury MM, Alsubhi NH, et al. Vitis vinifera seed extract ameliorates streptozotocin-induced pancreatic dysfunction by reducing interleukin-1Î² and up-regulation of omentin-1 mRNA. Trop J Pharm Res 2023; 22(2):289-295 doi: 10.4314/tjpr.v22i2.10

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of Vitis vinifera ethanol seed extract (SE) on pancreatic function markers associated with streptozotocin-induced diabetes in rats.

Methods: Non-diabetic rats (n = 12) were divided into 2 groups (GRPs); GRP1 served as the control, and GRP2 was administered orally with V. vinifera SE (500 mg/kg). Diabetic rats were divided into 2 GRPs; GRP3 served as the streptozotocin-induced diabetic rats, and GRP4 diabetic rats received V. vinifera SE (500 mg/kg). Blood was collected at the end of 4 weeks, and serum glucose and lipid profile were measured using colorimetric enzymatic kits. Serum insulin, and interleukin-1 beta (IL-1β) levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Histopathological alterations in pancreas were determined, while omentin-1 mRNA expression was investigated using quantitative real time-polymerase chain reaction (qRT-PCR).

Results: V. vinifera SE protected pancreatic function in diabetic rats. V. vinifera SE significantly increased serum insulin and HDL (p < 0.001), besides significantly decreasing serum glucose, cholesterol, triglyceride, and LDL (p < 0.001). Pathological studies also showed that V. vinifera SE preserved the pancreatic β cells’ shape, size, and cell population in the diabetic GRP. The extract significantly decreased serum IL-1β levels in diabetic rats (p < 0.001). Besides, the data demonstrated a significant up-regulation in omentin-1 mRNA expression in diabetic rats treated with V. vinifera SE.

Conclusion: V. vinifera SE has anti-diabetic and anti-hyperlipidemic properties in streptozotocin-induced rat model. The underlying mechanism of action might be a decrease in the pro-inflammatory cytokine (IL-1β) and increase in omentin mRNA. Clinical trials are required to validate these findings.

Keywords: Diabetes mellitus, Omentin-1, Interleukin-1Î², Vitis vinifera, Hyperlipidemia