Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Ganjiang Huangqin Huanglian Renshen Decoction improves insulin sensitivity by regulating intestinal flora in rats with Type 2 diabetes mellitus

Zhiping Peng, Yuanjun Zheng

Department of Geratology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, No. 453 Tiyuchang Road, Hangzhou, Zhejiang 310007, China;

For correspondence:- Yuanjun Zheng Email: 13003617778@163.com Tel:+8657185827720

Accepted: 28 March 2023 Published: 29 April 2023

Citation: Peng Z, Zheng Y. Ganjiang Huangqin Huanglian Renshen Decoction improves insulin sensitivity by regulating intestinal flora in rats with Type 2 diabetes mellitus. Trop J Pharm Res 2023; 22(4):833-839 doi: 10.4314/tjpr.v22i4.16

© 2023 The authors.
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Abstract

Purpose: To study the effect of Ganjiang Huangqin Huanglian Renshen Decoction (GHHRD) on intestinal microflora and insulin resistance (IR) in type 2 diabetes mellitus (T2DM) rats.

Methods: Streptozotocin (STZ) injection was used to establish a rat model of T2DM. The T2DM rats were divided into 5 groups: control, T2DM, T2DM + low-dose traditional Chinese medicine (TCM), T2DM + high-dose TCM, and T2DM + metformin groups. Total DNA kit was used for extraction of total DNA from fecal microorganisms from the T2DM model rats. High-throughput 16S rDNA sequencing was carried out to identify the intestinal flora of T2DM model rats. Fasting plasma glucose (FPG) and fasting serum insulin (FINS) levels were determined by a biochemical analyzer.

Results: The 16S rDNA sequencing analysis showed that after GHHRD treatment, the levels of Ruminococcus, Prevotellaceae, Escherichia-Shigella and Bacteroidota in the intestinal tract of T2DM model rats exhibited significant changes. Furthermore, GHHRD decreased the Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) index and IR in T2DM model rats.

Conclusion: GHHRD mitigates IR in T2DM model rats through alterations in the intestinal flora of the rats. This provides a theoretical basis for the potential use of GHHRD in the clinical management of T2DM.

Keywords: Type 2 diabetes mellitus (T2DM), Ganjiang Huangqin Huanglian Renshen Decoction (GHHRD), Intestinal microflora, Escherichia-Shigella, Insulin resistanc