Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Expressions and significance of 25 (OH) D, KL-6 and IL-32 in serum and alveolar lavage fluid in patients with connective tissue disease-related interstitial lung disease

Yulin Fang¹, Weiqing Li¹

¹Department of Geriatrics, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, Hubei 430015, China; ²Department of Traditional Chinese Medicine, Shenzhen Luohu Peopleâ€™s Hospital, Shenzhen, Guangdong 518001, China.

For correspondence:- Weiqing Li Email: liweiqing7532@tom.com Tel:+8675525650005

Accepted: 25 May 2023 Published: 30 June 2023

Citation: Fang Y, Li W. Expressions and significance of 25 (OH) D, KL-6 and IL-32 in serum and alveolar lavage fluid in patients with connective tissue disease-related interstitial lung disease. Trop J Pharm Res 2023; 22(6):1283-1289 doi: 10.4314/tjpr.v22i6.18

© 2023 The authors.
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Abstract

Purpose: To investigate the expressions and significance of 25 hydroxyvitamin D [25(OH)D3], Krebs von den Lungen (KL)-6, and interleukin (IL)-32 in serum and alveolar lavage fluid (SALF) in patients with connective tissue disease-related interstitial lung disease (CTD-ILD).

Methods: Forty CTD-ILD patients admitted to Affiliated Hospital of Jianghan University (The Sixth Hospital of Wuhan) were retrospectively categorized into a study group (SG), while 45 healthy individuals were categorized into a control group (CG). The SG was grouped based on the imaging performance of the high-resolution computed tomography (HRCT). If the lesions were ground-glass-like changes, featuring predominant exudative changes in spots and patchy shadows, the patients were placed in subgroup A of the study group, but if they were fibrous cord-like, grid-like, and honeycomb-like fibrotic lesions, the patients were placed in subgroup B of study group.

Results: Serum 25(OH)D3 in the SG was lower than in the CG, while the serum levels of KL-6 and IL-32 were higher than in CG. Furthermore, in the SG, KL-6 and IL-32 levels were lower in serum than in alveolar lavage fluid (p < 0.05). Sub-group A showed higher SALF levels in KL-6 and IL-32 than sub-group B. Forced vital capacity (FVC) and the diffusing capacity of the lungs for carbon monoxide (DLCO) in SG were lower than in CG (p < 0.05). Serum 25(OH)D3 had negative correlation with the SALF of KL-6 and IL-32 in CTD-ILD patients (p < 0.05), while KL-6 and IL-32 levels in serum were positively correlated with those in alveolar lavage fluid (p < 0.05).

Conclusion: The results indicate the possible involvement of 25(OH)D3, KL-6, and IL-32 in the development of CTD-ILD. Thus, serum 25(OH)D3, SALF KL-6, and IL-32 levels may affect lung function and therefore, can serve as indicators of the patients' condition.

Keywords: Connective tissue disease-related interstitial lung disease (CTD-ILD), Peripheral venous blood, Alveolar lavage fluid, 25 Hydroxyvitamin D, Krebs von