Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of zinc on weight gain, hematological parameters and tissue structure of the liver, kidney and spleen in albino mice

Tri Truong Van, Nhi Ho Linh Kieu, Huyen Nguyen Thi Thuong

Department of Human and Animal Physiology, Biology Faculty, Ho Chi Minh City University of Education, Ho Chi Minh City, Vietnam;

For correspondence:- Huyen Thuong Email: huyenntth@hcmue.edu.vn Tel:+84918605081

Accepted: 17 June 2023 Published: 31 July 2023

Citation: Van TT, Kieu NH, Thuong HN. Effect of zinc on weight gain, hematological parameters and tissue structure of the liver, kidney and spleen in albino mice. Trop J Pharm Res 2023; 22(7):1403-1409 doi: 10.4314/tjpr.v22i7.7

© 2023 The authors.
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Abstract

Purpose: To investigate the effect of varying zinc concentrations on weight gain, hematological parameters, and tissue structure of the liver, kidney, and spleen in albino mice.

Methods: The mice were administered daily zinc at 50, 70, and 90 mg/kg body weight. Weight gain was measured every two weeks. Hematological parameters were evaluated after 4 and 8 weeks, while biomolecular parameters were assessed after 8 weeks. Liver, kidney, and spleen were also assessed after 8 weeks using H & E staining procedures.

Results: Albino mice exhibited reduced weight gain, and altered hematological parameters after 8 weeks of exposure to varying zinc concentrations, with the highest dose of 90 mg/kg body weight having the most significant impact. Hematological parameters including blood cells, hemoglobin, hematocrit, blood clotting time, aspartate aminotransferase, and alanine transaminase decreased, while bilirubin, urea, and mean corpuscular hemoglobin concentration increased. Liver and kidney tissues exhibited widespread destruction such as necrosis and hemorrhage, while red pulp invaded white pulp activity in the spleen.

Conclusion: High levels of zinc from zinc chloride (90 mg/kg) affect body weight, and hematological parameters, as well as liver and kidney histology in albino mice. The most significant negative effects include the potential toxicity and adverse health consequences of excessive zinc intake. Consequently, this study provides the groundwork for investigating natural compounds that inhibit zinc toxicity.

Keywords: Hematology, Kidney, Liver, Spleen, Zinc