Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Embelin enhances the osteogenic potential of LPS-induced periodontal ligament stem cells by activating AMPK and SIRT1

Lijian Wang^1,2, Weidong Wu² , Xiaoying Wei²

¹Graduate School, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 310053, China; ²Department of Stomatology, Lishui People's Hospital, Lishui, Zhejiang Province 323000, China.

For correspondence:- Weidong Wu Email: wuweidong3022801@126.com Tel:+8618957093307

Accepted: 27 July 2023 Published: 31 August 2023

Citation: Wang L, Wu W, Wei X. Embelin enhances the osteogenic potential of LPS-induced periodontal ligament stem cells by activating AMPK and SIRT1. Trop J Pharm Res 2023; 22(8):1561-1566 doi: 10.4314/tjpr.v22i8.5

© 2023 The authors.
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Abstract

Purpose: To investigate the role of embelin in periodontal ligament stem cells (PDLSCs) in an in vitro model of periodontitis.

Methods: Lipopolysaccharide (LPS)-stimulated PDLSCs was used to construct a periodontitis cell model. PDLSCs in the treatment group were pretreated with different concentrations of Embelin, and CCK-8 and TUNEL staining were used to analyze cell viability and apoptosis. Enzyme-linked immunosorbent assay (ELISA) kits were used to evaluate the levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, and MCP-1) while reactive oxygen species levels were assessed by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. Subsequently, osteogenic marker, ALP activity and protein expression levels of Runx2, OCN and BMP-2 in PDLSCs were evaluated by western-blot assay; AMPK and SIRT1 levels were also determined using Western blot assay.

Results: Embelin pretreatment inhibited PDLSCs apoptosis, inflammatory factors, and oxidative stress, but up-regulated ALP, Runx2, OCN, and BMP-2 levels (p < 0.05). In addition, AMPK phosphorylation and SIRT1 protein levels were regulated by embelin (p < 0.05).

Conclusion: Embelin exerts anti-inflammatory, anti-oxidative and osteogenic differentiation effects in LPS-induced PDLSCs cells in vitro by activating AMPK/SIRT1 signaling. Therefore, the compound has potentials for use in the management of periodontitis

Keywords: Periodontitis, PDLSCs, Embelin, Osteogenic differentiation, AMPK, SIRT1