Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Supercritical fluid carbon dioxide extract of Alpinia oxyphylla ameliorates dextran sulfate sodium-induced intestinal barrier damage in mice

Fan Yang¹, Yongqing Tao¹ , Chen Chen¹, Wei Zhao¹, Juan Wang¹, Beibei Peng¹, Ke Lv¹, Patrick O Erah² , Hui Zhao¹

¹Tianjin Key Laboratory of Food and Biotechnology, Tianjin International Joint Center of Food Science and Engineering, State Experimental and Training Centre of Food and Drug, School of Biotechnology and Food Science, Tianjin University of Commerce, No. 409 Guangrong Road, Tianjin, 300134 China; ²Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.

For correspondence:- Yongqing Tao Email: tyqing@tjcu.edu.cn

Accepted: 1 May 2024 Published: 29 May 2024

Citation: Yang F, Tao Y, Chen C, Zhao W, Wang J, Peng B, et al. Supercritical fluid carbon dioxide extract of Alpinia oxyphylla ameliorates dextran sulfate sodium-induced intestinal barrier damage in mice. Trop J Pharm Res 2024; 23(5):833-845 doi: 10.4314/tjpr.v23i5.5

© 2024 The authors.
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Abstract

Purpose: Inflammation and oxidative stress are the leading causes of intestinal barrier dysfunction and a wide range of diseases. The purpose of this study was to explore the protective effect and elucidate potential mechanisms of supercritical carbon dioxide extract from Alpinia oxyphylla Miq. (AOE) on intestinal inflammation.

Methods: The AOE was extracted by supercritical carbon dioxide extraction and its components were determined. Cytotoxicity of the extract (0.05 to 20μg/mL) was determined on Caco-2 cells. In vitro assessment of whether AOE protected against LPS-induced intestinal barrier dysfunction was done on Caco-2 cells pretreated with non-cytotoxic concentrations (0.5μg/mL and 1 μg/mL) of the extract. Experimental colitis of mouse model was induced by drinking water containing 3% dextran sulfate sodium (DSS). The intestinal permeability was assessed by TEER and fluorescein isothiocyanate conjugated dextran (FITC). Pathological evidence and possible mechanism were verified by tissue staining and molecular methods including quantitative real-time polymerase chain reaction (qRT-PCR) and western blot.

Results: TEER and FITC testing indicated that AOE pretreatment significantly improved intestinal barrier hemostasis (p<0.05). Furthermore, AOE pretreatment counteracted DSS-induced upregulation of pro-inflammatory cytokines and oxidative stress (p<0.05). Lastly, two crucial signal pathways regarding hemostasis of intestinal barrier, NF-κB and NOX1-LCN2, were significantly attenuated upon AOE pretreatment (p<0.05).

Conclusion: This study sheds lights on the protective effect of AOE for intestinal hemostasis and supports the development of AOE-based intestinal protective agents.

Keywords: Alpinia oxyphylla extract; Intestinal inflammation; Dextran Sulfate Sodium Salt; NF-?B; Oxidative stress