Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Phytochemical screening and cytotoxic activities of Enhalus acoroides (L.f.) Royle and Halimeda macroloba Decaisne on cervical cancer cell lines

Raphatphorn Navakanitworakul¹, Kesara Nittayaboon¹, Parinuch Chumkaew², Sinjai Phetcharat³, Jaruwan Mayakun³

¹Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,; ²Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani campus, Surat Thani 84000,; ³Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.

For correspondence:- Jaruwan Mayakun Email: jaruwan.may@psu.ac.th

Received: 14 July 2024 Accepted: 7 December 2024 Published: 30 December 2024

Citation: Navakanitworakul R, Nittayaboon K, Chumkaew P, Phetcharat S, Mayakun J. Phytochemical screening and cytotoxic activities of Enhalus acoroides (L.f.) Royle and Halimeda macroloba Decaisne on cervical cancer cell lines. Trop J Pharm Res 2024; 23(12):2053-2057 doi: 10.4314/tjpr.v23i12.10

© 2024 The authors.
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Abstract

Purpose: To determine the cytotoxic effects of phytochemical compounds in Enhalus acoroides and Halimeda macroloba crude extracts on cervical carcinoma and normal kidney tissue cells. Methods: Dried Enhalus acoroides and Halimeda macroloba were macerated in ethanol. Then, phytochemical screening was done using thin-layer chromatography (TLC). The cytotoxic activities of extracts against HeLa and SiHa cervical cancer cells were performed using MTT assay. Results: The H. macroloba extract was highly active against SiHa cells, with an IC50 of 17.22 ± 3.93 µg/mL, and moderately active against HeLa cells, with an IC50 of 36.57 ± 7.26 µg/mL. The E. acoroides extract was moderately active against both HeLa and SiHa cells. Moreover, both extracts exhibited lower toxicity against HEK293 normal kidney cells, with IC50 values of > 80 µg/mL. Conclusion: These results suggest that E. acoroides and H. macroloba exert potent cytotoxic activity on cervical cancer cells. The mode of action associated with the cytotoxicity of these extracts, and the effect of the combination of the extracts with chemotherapy, should be investigated in subsequent studies.

Keywords: Cytotoxicity, Cancer cell, Phenolic compounds, Seagrass, Seaweed