Ramzi Amran 
,
Ahmad Alhimaidi,
Aiman Ammari
For correspondence:- Ramzi Amran Email: ramran@ksu.edu.sa
Received: 19 October 2024 Accepted: 13 January 2025 Published: 27 January 2025
Citation: Amran R, Alhimaidi A, Ammari A. Evaluation of the California mastitis test, pH and milk colour as indicators for subclinical mastitis detection in local dairy cows. Trop J Pharm Res 2025; 24(1):15-20 doi: 10.4314/tjpr.v24i1.3
© 2025 The authors.
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Purpose: To evaluate the utilization of diagnostic tools such as the California Mastitis Test (CMT) and milk pH and colour assessments for detecting subclinical mastitis (SCM). Method: A total of 60 milk samples were collected from 60 apparently healthy local dairy cows in Dhamar City, Yemen, to investigate the incidence of SCM. The samples were subjected to California Mastitis Test (CMT) as well as tests for pH and colour. Results: According to CMT results, 45 % of the cows had SCM. The rate of prevalence in (+), (++) and (+++) CMT results was 17 (28 %), 7 (12 %) and 3 (5 %), respectively. In terms of milk pH, 27 (45 %) of the samples had a pH between 6.6 and 6.8, indicating no signs of SCM. However, 20 (33 %) of the samples exhibited a pH < 6.6, reflecting increased acidity, while 13 (22 %) had a pH > 6.8, indicating alkaline milk, a marker of severe inflammation and tissue damage. The assessment of milk colour showed that 34 (57 %) of the samples had normal white milk, indicating no mastitis, while 17 (28 %) were yellow, suggesting mild to moderate inflammation or infection. Finally, 9 (15 %) of the samples were pink, indicating the presence of blood and severe inflammation with udder damage. Conclusion: These findings underscore the reliability of CMT, pH and milk colour assessments as effective diagnostic tools for detecting SCM in dairy cows, thus providing valuable insights for early intervention and management strategies.
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