Ni Luh Watiniasih1,
Dewa Ayu Swastini2,
Luh Putu Ida Harin3,
Komang Dian Aditya Putra4,
Putu Ayuning Dinda Nirmalayanthi4,
Sharon Grace Tarigan4,
Eka Indra Setyawan2 
For correspondence:- Eka Setyawan Email: ekaindrasetyawan@unud.ac.id
Received: 3 August 2024 Accepted: 16 January 2025 Published: 28 January 2025
Citation: Watiniasih NL, Swastini DA, Harin LP, Putra KD, Nirmalayanthi PA, Tarigan SG, et al. In vivo investigation of Roselle flower (Hibiscus sabdariffa L.) and Bee pollen (Tetragonula laeviceps) on carbon tetrachloride-induced spermatozoa damage. Trop J Pharm Res 2025; 24(1):77-83 doi: 10.4314/tjpr.v24i1.11
© 2025 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the effect of roselle flower and bee pollen on carbon tetrachloride (CCl4)- induced spermatozoa damage. Methods: Roselle flowers (Hibiscus sabdariffa L.) and bee pollen (Tetragonula laeviceps) were obtained from Bali, Indonesia. A total of 24 BALB/c (Mus musculus) mice were used and randomly assigned into control, negative control (CCl4-induced), positive control (CCl4 + ascorbic acid) and treatment groups (25, 50, and 100 mg/20 g). Spermatozoa-induced damage was done using carbon tetrachloride (CCl4). Sperm levels, movement, viability, and morphology of the spermatozoa were evaluated. Results: Roselle and bee pollen granules at 50 mg/20 g (11.2 mg of roselle extract and 28 mg of pollen extract), demonstrated the most significant improvement in spermatozoa levels, movement, viability, and morphological characteristics compared to negative controls (p < 0.05). Conclusion: Roselle and bee pollen granular combination offers a promising natural intervention for mitigating chemically induced reproductive toxicity. It suggests potential therapeutic applications in preserving male reproductive health and counteracting oxidative stress-related spermatogenic damage.
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