Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidant profiling and molecular characterization of novel Lacticaseibacillus rhamnosus strains isolated from human microbiota

Abdelmalek Chaalel¹ , Nawal Boukezzoula¹, Djilali Benabdelmoumene², Choukri Tefiani³, Mimoune Souna³, Mohamed-cherif Bentahar², Ghada Lamraoui³, Taleb Aridj¹

¹Laboratory of Beneficial Microorganisms, Functional Foods and Health, Faculty of Natural Sciences and Life, Abdelhamid Ibn Badis University of Mostaganem, Mostaganem 27000; ²Laboratory of Applied Animal Physiology, Abdelhamid Ibn Badis University of Mostaganem, BP 188, Mostaganem 27000; ³Laboratory of Functional Agrosystems & Technologies of Agronomic Sectors, University of Abou Bekr Belkaïd, Tlemcen 13000, Algeria.

For correspondence:- Abdelmalek Chaalel Email: abdelmalek.chaalel@univ-mosta.dz

Received: 18 January 2025 Accepted: 13 March 2025 Published: 30 March 2025

Citation: Chaalel A, Boukezzoula N, Benabdelmoumene D, Tefiani C, Souna M, Bentahar M, et al. Antioxidant profiling and molecular characterization of novel Lacticaseibacillus rhamnosus strains isolated from human microbiota. Trop J Pharm Res 2025; 24(3):337-343 doi: 10.4314/tjpr.v24i3.6

© 2025 The authors.
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Abstract

Purpose: To assess the antioxidant properties of two newly isolated Lacticaseibacillus rhamnosus strains (BVV1 and BBV2) from breastfed infants for potential use in nutritional and probiotic formulations. Methods: Strains were identified using the Basic Local Alignment Search Tool (BLAST) with the National Center for Biotechnology Information (NCBI) 16S database. The in vitro antioxidant activities were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging ability, Fe²? chelation, lipid peroxidation inhibition, and superoxide anion reduction compared to L. rhamnosus GG. Results: The BBV2 exhibited the highest DPPH scavenging (82.44 ± 1.87 %) and Fe²? chelation (71.72 ± 2.78 %). Hydroxyl radical scavenging was strongest in GG (83.12 ± 2.59 %) than in BBV2 (79.59 ± 4.49 %). Lipid peroxidation inhibition in GG (63.44 ± 4.66 %) was higher than in BBV2 (58.24 ± 1.38 %). Conclusion: BBV2 shows strong antioxidant potential, making it a promising natural alternative to synthetic antioxidants in probiotic and nutritional applications.

Keywords: Lacticaseibacillus rhamnosus, Breastfed infants, Microbiota, Probiotic, Antioxidant