Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Development of three methods for simultaneous quantitative determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in oral liquids

Hany W Darwish^1,2 , Fadia H Metwally^2,3, Abdelaziz El Bayoumi²

¹Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia; ²Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, ET 11562, Cairo, Egypt; ³Ibn Sina National College for Medical Studies, AlMahjer Road, Jeddah, Saudi Arabia.

For correspondence:- Hany Darwish Email: hdarwish75@yahoo.com,hdarwish@ksu.edu.sa Tel:+96614677343

Received: 22 October 2014 Revised: 18 December 2014 Published: 30 January 2015

Citation: Darwish HW, Metwally FH, Bayoumi AE. Development of three methods for simultaneous quantitative determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in oral liquids. Trop J Pharm Res 2015; 14(1):153-161 doi: 10.4314/tjpr.v14i1.22

© 2015 The authors.
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Abstract

Purpose:To develop new selective, precise, and accurate methods for the simultaneous determination of chlorpheniramine maleate (CHP) and dexamethasone (DX) in the presence of methyl and propyl paraben in phenadone syrup.

Methods:In the first two methods, the predictive abilities of principal component regression (PCR) and partial least squares (PLS), respectively, were examined for the analysis of the quaternary mixture. The third method, high performance thin layer chromatography (HPTLC)-densitometric method, was based on the separation of the mixture on silica gel plates using chloroform: methanol (93:7, v/v) as a mobile phase.

Results:All the proposed methods were successfully applied to the analysis of raw materials and dosage form. For PCR method, recovery of chlorpheniramine maleate and dexamethasone in the dosage form was 98.89 ± 1.736 and 102.36 ± 1.86 %, respectively while for. PLS method, recovery of chlorpheniramine maleate and dexamethasone was 98.94 ± 1.69 and 102.33 ±1.84, respectively. On the other hand, recovery of the two analytes by HPTLC method was 100.72 ± 1.05 and 102.29 ± 3.98, respectively. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by a reported HPLC method.

Conclusion:The proposed methods are fast, accurate and specific, and can be applied for the quantitative determination of the two analytes without interference from added excipient, thus obviating the need for preliminary extraction of analytes from the pharmaceutical formulation. Thus, they are suitable for use in quality control (QC) laboratories and pharmaceutical industry.

Keywords: Chlorpheniramine maleate, Dexamethasone, Principal component regression, Partial least squares, High performance thin layer chromatography, Excipients