Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Enhancement of diosgenin production in plantlet and cell cultures of Dioscorea zingiberensis by Palmarumycin C13 from the endophytic fungus, Berkleasmium sp. Dzf12

Yan Mou, Kaiyi Zhou, Dan Xu, Ruiting Yu, Jing Li, Chunhui Yin, Ligang Zhou

MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;

For correspondence:- Ligang Zhou Email: lgzhou@cau.edu.cn Tel:+861062731199

Received: 7 October 2014 Revised: 7 January 2015 Published: 28 February 2015

Citation: Mou Y, Zhou K, Xu D, Yu R, Li J, Yin C, et al. Enhancement of diosgenin production in plantlet and cell cultures of Dioscorea zingiberensis by Palmarumycin C13 from the endophytic fungus, Berkleasmium sp. Dzf12. Trop J Pharm Res 2015; 14(2):241-248 doi: 10.4314/tjpr.v14i2.8

© 2015 The authors.
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Abstract

Purpose: To study the effect of palmarumycin C₁₃, an elicitor from the endophytic fungus Berkleasmium sp. Dzf12, on growth and diosgenin production in plantlet or cell cultures of its host plant, Dioscorea zingiberensis.

Methods: Palmarumycin C₁₃ was isolated from the ethyl acetate extract of the endophytic fungus Berkleasmium sp. Dzf12 using a combination of high-speed counter-current chromatography (HSCCC), Sephadex LH-20 chromatography and preparative high performance liquid chromatography (HPLC). The biomass of the plantlet and cell cultures of D. zingiberensis as well as their diosgenin content and yield were analyzed after treatment with palmarumycin C₁₃.

Results: Optimal elicitation of diosgenin production by palmarumycin C₁₃ in D. zingiberensis plantlet and cell cultures was achieved when palmarumycin C₁₃ was added to the medium at a concentration of 60 mg/L (for plantlet culture) at the beginning of culturing or 10 mg/L (for cell culture) on day 25 after inoculation. By using these optimal concentrations, the diosgenin yield of the cultured plantlets reached its maximum of 6.44 mg/L, that is, > 1.4-fold increase, while diosgenin yield of the cultured cells reached a maximum of 10.73 mg/L, which is an > 8.0-fold increase.

Conclusion: Addition of palmarumycin C₁₃ from the endophytic fungus, Berkleasmium sp. Dzf12, is a potentially effective strategy for enhancing diosgenin production in D. zingiberensis plantlet and cell cultures.

Keywords: Endophytic fungus, Berkleasmium sp. Dzf12, Spirobisnaphthalene, Palmarumycin C13, Dioscorea zingiberensis, Diosgenin, Elicitation, Cell culture, Plant