Hai-ming Chen1,2,
Lan Wang2,
Xiao-li Wu1,3,
Chu-wen Li1,4,
You-liang Xie1,
Yu-hong Liu1,
Yong-zhuo Liang1,
Xiao-ying Chen1,
Xiao-ping Lai1,5,
Jian-nan Chen1,5,
Yu-cui Li1 
,
Zi-ren Su1,5
For correspondence:- Yu-cui Li Email: liyucui@gzucm.edu.cn
Received: 11 October 2014 Revised: 12 January 2015 Published: 28 February 2015
Citation: Chen H, Wang L, Wu X, Li C, Xie Y, Liu Y, et al. Determination and distribution study of pogostone in rat tissues by ultra-fast liquid chromatography. Trop J Pharm Res 2015; 14(2):279-286 doi: 10.4314/tjpr.v14i2.13
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To develop and validate a rapid, sensitive and reliable ultra-fast liquid chromatography (UFLC) method with photodiode array (PDA) detection for the determination of pogostone (PO) in rat tissues using honokiol as internal standard (IS).
Methods: Rats were randomly divided into two groups (intravenous administration group and oral administration group) and given of a single dose of 10 mg/kg PO by intravenous administration and oral administration, respectively. After intravenous injection, the rats were sacrificed at 15, 60 and 360 min, while rats, after oral administration, were euthanasized at 30, 90 and 360 min, respectively. For the analysis of the preparation, optimal chromatographic conditions were determined using Acquity UPLC BEH C18 column with acetonitrile-water containing 0.1 % formic acid (55:45, v/v) as the mobile phase, at a flow rate of 400 µL/min. UV detection wavelength was set at 310 nm with temperature maintained at 30 °C.
Results: Good linear relationship of calibration curve (r > 0.9984) was achieved over the range of 0.1 - 40 μg/mL for all the tissue samples. The limit of quantification (LOQ) and limit of detection (LOD) were 0.1 and 0.05 μg/mL, respectively. This method proved to have good precision, accuracy, stability, extraction recovery and matrix effect for tissue distribution studies of PO in rats.
Conclusion: The developed method is suitable for tissue distribution studies in rats following intravenous and oral administration of PO at a dose of 10 mg/kg.
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