Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Enhanced production of palmarumycins C12 and C13 in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 with in situ macroporous resin adsorption

Yan Mou, Jing Li, Kaiyi Zhou, Ruiting Yu, Dan Xu, Haiyu Luo, Daiwan Lai, Ligang Zhou

MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;

For correspondence:- Ligang Zhou Email: lgzhou@cau.edu.cn Tel:+861062731199

Received: 28 November 2014 Accepted: 23 February 2015 Published: 31 March 2015

Citation: Mou Y, Li J, Zhou K, Yu R, Xu D, Luo H, et al. Enhanced production of palmarumycins C12 and C13 in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 with in situ macroporous resin adsorption. Trop J Pharm Res 2015; 14(3):407-414 doi: 10.4314/tjpr.v14i3.8

© 2015 The authors.
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Abstract

Purpose: To evaluate in situ macroporous resin adsorption for enhancement of palmarumycins C₁₂ and C₁₃ production in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12.

Methods: Ten macroporous adsorption resins (D-101, D1300, HPD-100, X-5, AB-8, DM130, ADS-17, DA-201, NKA-9 and S-8) were tested for absorption of palmarumycins C₁₂ and C₁₃ in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12.

Results: Among the resins, DA-201 showed the most significant enhancing effect on accumulation of palmarumycins C₁₂ and C₁₃ in mycelial liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When resin DA-201 was applied to the medium at a concentration of 4.17 % on day 11 and then harvested on day 15, the maximal yield of palmarumycins C₁₂ and C₁₃ was 149.86 and 55.78 mg/L, which correspond to 70.69- and 1.82-fold higher than for control (2.12 and 30.70 mg/L, respectively). Approximately 95.81 % of palmarumycin C₁₂ and 87.20 % of palmarumycin C₁₃ were distributed in resin DA-201.

Conclusion: The results indicate that in situ resin adsorption is an effective strategy for enhancing the production of palmarumycins C₁₂ and C₁₃, and also for facilitating their recovery from the mycelial liquid culture of Berkleasmium sp. Dzf12.

Keywords: Endophytic fungus, Berkleasmium sp. Dzf12, Spirobisnaphthalene, Palmarumycin, Macroporous adsorption resins, Mycelial liquid culture