Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Quantitative variations of CD4+CD25+ cells in Peking duck-white leghorn chimeras based on bone marrow mesenchymal stem cells

Xinxin Qin, Lei Rui, Wenting Zhang, Zhuyu Qiu, Zandong Li

State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China;

For correspondence:- Zandong Li Email: lzdws@cau.edu.cn Tel:+861062732144

Received: 6 January 2017 Accepted: 21 March 2017 Published: 29 April 2017

Citation: Qin X, Rui L, Zhang W, Qiu Z, Li Z. Quantitative variations of CD4+CD25+ cells in Peking duck-white leghorn chimeras based on bone marrow mesenchymal stem cells. Trop J Pharm Res 2017; 16(4):761-769 doi: 10.4314/tjpr.v16i4.4

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To develop a chimera via microinjection of poultry xenogeneic bone marrow mesenchymal stem cells (BMMSCs), and to assess its immune tolerance based on variations in proportion of CD4⁺CD25⁺ cells in CD4⁺ cells (specific CD4⁺CD25⁺ cells).

Methods: BMMSCs were flush out from femurs and tibias of Peking ducks with phosphate-buffered saline and cultured. Their morphology was determined with a microscope. Several surface markers (i.e., CD44, CD45, CD71, CD73 and CD34) were used to identify the cells.

Results: The results indicate successful chimera development. CD4⁺CD25⁺ cells derived from the thymus of chimeras were migrated to the spleen and cecal tonsils. This migration was more obvious in chimeras than in the control group, suggesting a more robust immune system in the chimeras. The migration tendency gradually decreased with time. There were significant increases in specific CD4⁺CD25⁺ cells, TGF-β and IL-10 in cecal tonsils throughout the experimental period (30 days). However, in thymus and spleen, variations in specific CD4⁺CD25⁺ cells were observed only on the 1st day post-hatching.

Conclusion: The results suggest a relatively pure BMMSC population without contaminating hematopoietic stem cells. Differentiation of the BMMSCs into osteoblasts and adipocytes was inducible, indicating typical MSC character

Keywords: Bone marrow mesenchymal stem cells, Immune tolerance, Chimera, Specific CD4+CD25+ cells, Cell migration