Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro protective effect of ganoderol A isolated from ganadermalucidum against ultraviolet A radiation and its anti-inflammatory properties

Fujun Jin¹, Hongwei Zhao², Xiao Yuan¹, Shiyu Zou², Qiaoli Wang¹, Chungwah Ma², Zhe Ren¹ , Yifei Wang¹

¹Guangzhou Jinan Biomedicine Research and Development Center, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632; ²Infinitus (China) Company Co, Ltd, Guangzhou 510632, China.

For correspondence:- Yifei Wang Email: twang-yf@163.com Tel:+862085223426

Received: 17 April 2014 Accepted: 16 January 2015 Published: 31 March 2015

Citation: Jin F, Zhao H, Yuan X, Zou S, Wang Q, Ma C, et al. In vitro protective effect of ganoderol A isolated from ganadermalucidum against ultraviolet A radiation and its anti-inflammatory properties. Trop J Pharm Res 2015; 14(3):415-421 doi: 10.4314/tjpr.v14i3.9

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the ultraviolet A (UVA) protection and anti-inflammatory activity of ganoderol A extracted from Ganodermalucidum.

Methods: The cytotoxicity and in vitro protective effect of ganoderol A against UVA damage were evaluated by MTT assay. Apoptosis and cell-cycle arrest of NIH/3T3 fibroblast cells were assayed by fluorescence-activated cell sorting (FCS). expression of monocyte chemotactic protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) were determined using quantitative real-time polymerase chain reaction (qPCR).

Results: The results indicate that the maximal non-toxic concentration of ganoderol A in NIH/3T3 cells and RAW 264.7 macrophages was 50 and 25 µg/mL respectively. DNA in the tails and tail length decreased by 55 and 70 %, respectively, in the group pretreated with ganoderol A compared with the UVA-treated group. G1 phase cells decreased by 23 %, whereas the number of apoptotic cells returned to normal. The expression of MCP-1 and iNOS declined to 60 and 15 %, respectively, compared with LPS-stimulated group.

Conclusion: Ganoderol A has significant anti-inflammatory activity and protection against UVA damage, thus suggesting that the compound is a candidate for the development of a suitable product to protect skin from UV-induced photoaging.

Keywords: Anti-ultraviolet A, Anti-inflammatory, Ganoderol A, Ganodermalucidum, Photoaging