Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Lycium barbarum polysaccharide attenuates cisplatin-induced apoptosis in ovary granulosa cells via alleviation of endoplasmic reticulum stress

Li-Qiong Huang^1,2, Yuan-Zhen Zhang^1,2 , Bo Zheng³, Yi He³

¹Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University; ²Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University, Wuhan 430071; ³Department of Obstetrics and Gynecology, Xianning Central Hospital, Xianning, Hubei, 437000, PR China.

For correspondence:- Yuan-Zhen Zhang Email: YZZhang21@163.com Tel:+8602767812888

Received: 25 October 2016 Accepted: 8 March 2017 Published: 30 April 2017

Citation: Huang L, Zhang Y, Zheng B, He Y. Lycium barbarum polysaccharide attenuates cisplatin-induced apoptosis in ovary granulosa cells via alleviation of endoplasmic reticulum stress. Trop J Pharm Res 2017; 16(4):827-835 doi: 10.4314/tjpr.v16i4.12

© 2017 The authors.
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Abstract

Purpose: To investigate the effect of Lycium barbarum polysaccharide (LBP) on apoptosis of ovary granulose cells (GCs), as well as its underlying mechanism.

Methods: GCs were isolated from Sprague Dawley (SD) rats and divided into three groups: control group, model group (DDP) and LBP group. Cell morphology was observed by H & E staining under optical microscope. expression of follicle stimulating hormone receptor (FSHR) was assessed by immunohistochemistry (IHC), while cell viability was assayed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT). Apoptosis was determined by flow cytometry. expressions of glucose-regulated protein 78(GRP78), C/EBP homologous protein (CHOP), caspase-3, Bax protein and B cell lymphoma-2(Bcl-2) were assayed by Western blot and qRT-PCR.

Results: Apoptosis index (37.6 ± 2.44 %) was significantly higher (p > 0.05) in DDP group than in the control group (14.3 ± 1.09 %), while mRNA levels and expressions of caspase-3, Bcl-2 and Bax increased significantly (p > 0.05). expressions of GRP78 and CHOP in the DDP group were also higher than in the control group (p > 0.05). However, these effects were effectively blocked by co-incubation with LBP. Moreover, the DDP-induced increase in apoptosis index was dose-dependently and significantly lowered by LBP (p > 0.05).

Conclusion: LBP exerts protective effect on cisplatin-induced apoptosis in ovary granulosa cells by alleviating endoplasmic reticulum stress and regulating levels of apoptosis-related proteins. Thus, LBP has the potential for alleviating adverse effects induced by cisplatin in the treatment of ovary granulosa lesions

Keywords: Cis-platin, Ovary granulosa lesions, Lycium barbarum polysaccharide, Endoplasmic reticulum stress, Apoptosis