Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Assessment of absorption of four lignan constituents of JingNing particles in rat gut using in situ single-pass intestinal perfusion

Chunjing Yang¹, XingBin Yin¹, Xiaoxv Dong¹, Jing Fu¹, Wenping Wang¹, Xueying Du¹, Long tai You¹, Lin Guo¹, Sali Cao¹, Huyiligeqi .^1,2, Jian Ni¹

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102; ²Affiliated Hospital, Inner Mongolia University for Nationalities, Tongliao 028000, China.

For correspondence:- Jian Ni Email: njtcm@263.net

Received: 16 November 2016 Accepted: 15 March 2017 Published: 30 April 2017

Citation: Yang C, Yin X, Dong X, Fu J, Wang W, Du X, et al. Assessment of absorption of four lignan constituents of JingNing particles in rat gut using in situ single-pass intestinal perfusion. Trop J Pharm Res 2017; 16(4):837-847 doi: 10.4314/tjpr.v16i4.13

© 2017 The authors.
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Abstract

Purpose: To study small intestinal absorption of schisadrol A, schisandrol B, schizandrin A and schisandrin B in JingNing particles using in situ single-pass intestinal perfusion (SPIP).

Methods: Absorption rate constant (Ka) and apparent permeability (Papp) of the drugs at different concentrations in various parts of rat small intestine (duodenum, jejunum and ileum) were determined using SPIP. JingNing particles were also perfused in situ at different pH in the entire rat intestine. Ethanol extract of Schisandra chinensis (standard) at low concentration was perfused in the duodenum for comparison with extract of JingNing particles.

Results: The order of apparent permeability of the four lignans was schisandrol A < schisandrol B < schizandrin A < schisandrin B. Ka and Papp values of the four lignans in JingNing particles were concentration-dependent. Absorption increased in the rank order: ileum > duodenum > jejunum. Optimum absorption pH was 6.50. Polygala tenuifolia extract and volatile oil of Rhizoma acori tatarinowii significantly (p < 0.05) enhanced the absorption of the four lignans.

Conclusion: The four lignans were well absorbed in the intestinal tract, particularly the ileum, probably through carrier-mediated transport. The alcohol extract of Polygala tenuifolia and volatile oil of Rhizoma acori graminei enhanced the absorption of the four lignans

Keywords: JingNing, Intestinal absorption, Polygala tenuifolia, Rhizoma acori graminei, Lignans, Schisandrol, Schisandrin, Single-pass intestinal perfusion