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Original Research Article | OPEN ACCESS

Effect of a heme oxygenase-1 inducer on NADPH oxidase expression in alcohol-induced liver injury in male Wistar rats

Phanit Koomhin1,2, Chuchard Punsawad1, Prasit Suwannalert3, Sarawoot Palipoch2

1School of Medicine; 2The Pathobiology of Cell and Tissue Research Group, Walailak University, Nakhon Si Thammarat, 80161; 3Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

For correspondence:-  Sarawoot Palipoch   Email: sarawoot.pa@wu.ac.th   Tel:+6675672873

Received: 16 November 2016        Accepted: 7 April 2017        Published: 30 May 2017

Citation: Koomhin P, Punsawad C, Suwannalert P, Palipoch S. Effect of a heme oxygenase-1 inducer on NADPH oxidase expression in alcohol-induced liver injury in male Wistar rats. Trop J Pharm Res 2017; 16(5):1039-1044 doi: 10.4314/tjpr.v16i5.10

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigated the effect of hemin, a heme oxygenase-1 (HO-1) inducer, on nicotinamide adenine dinucleotide phosphate oxidase (NOX) expression in rats with alcohol-induced liver injury.
Methods: Male Wistar rats were randomly divided into four groups consisting of the control group, the ethanol (EtOH) group, the EtOH + zinc protoporphyrin IX (ZnPP-IX) group and EtOH + hemin group. Hepatic NOX gene expression and immunohistochemistry of hepatic NOX1 and NOX4 were investigated in week 4.
Results: EtOH significantly increased levels of NOX. An immunohistochemical study demonstrated a high number of immunopositive hepatocytes for NOX1 in the EtOH group and EtOH + ZnPP-IX group compared with the control group. Hemin administration downregulated NOX gene expression and lowered the number of immunopositive hepatocytes for NOX1. In contrast, ZnPP-IX (HO-1 inhibitor) administration caused upregulation of NOX gene expression and increased the number of immunopositive hepatocytes for NOX1.
Conclusion:  HO-1 inducer, hemin, alleviates oxidative stress-induced alcoholic liver injury by reducing NOX, especially NOX1

Keywords: NADPH oxidase, Immunohistochemistry, Heme oxygenase-1, Hemin, Reactive oxygen species, Alcohol-induced liver disease

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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