Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cardioprotective effect of Shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload

Zhi-Hua Wang¹, Jian-Hao Pan¹, Xian-Peng Ma², Xiao-Yun Xu¹, Wen-Hui Yu¹, Wen-Juan Fu¹, Jian Fe¹, Chang-Qiong Bi², Wei Wei², Qu Zhao², Feng Wang¹, Dan Tang¹, Kaihe Ye¹, Zhixian Yi³, Hong Nie¹

¹Guangdong Province Key Laboratory of Pharmacodynamics Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, 510632, China; ²Guizhou Jingfeng Injection Co. Ltd, No. 158 Gaoxin Road Wudang District, Guiyang, 550018, China; ³School of Information Studies, Charles Sturt University, Locked Bag 588, Boorooma Street, Wagga Wagga, NSW 2678, Australia.

For correspondence:- Hong Nie Email: tnieh@jnu.edu.cn Tel:+862085222810

Received: 19 December 2016 Accepted: 19 April 2017 Published: 30 May 2017

Citation: Wang Z, Pan J, Ma X, Xu X, Yu W, Fu W, et al. Cardioprotective effect of Shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload. Trop J Pharm Res 2017; 16(5):1097-1104 doi: 10.4314/tjpr.v16i5.18

© 2017 The authors.
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Abstract

Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose Injection (SGI) in rat acute myocardial infarction (AMI).

Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’ consecutive intravenous administration of SGI, serum samples were used to conduct biochemical analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology and qPCR studies. Intracellular Ca²⁺ {(Ca²⁺)i} overload in H9c2 cells was measured by laser scanning confocal microscope (LSCM).

Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram (ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression, but increased Ca²⁺-Mg²⁺-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca²⁺)i overload to protect cells.

Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial infarct size, restoring ST segment and reversing (Ca²⁺)i overload. It suggests that SGI may be a new clinical candidate to treat myocardial infarction

Keywords: Shenxiong glucose injection, Tanshinol, Ligustrazine, Myocardial infarction, Intracellular Ca2+ overload, Calmodulin, Calmodulin-dependent protein kin