Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of antioxidant and anti-neuroinflammatory activities of Hizikia fusiformis (Harvey) Okamura extract

Sung-Gyu Lee, Hyun Kang

Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam, 330-714, Republic of Korea;

For correspondence:- Hyun Kang Email: hyunbio@gmail.com, hkang@dankook.ac.kr Tel:+82415501452

Received: 18 December 2014 Accepted: 27 February 2015 Published: 31 March 2015

Citation: Lee S, Kang H. Evaluation of antioxidant and anti-neuroinflammatory activities of Hizikia fusiformis (Harvey) Okamura extract. Trop J Pharm Res 2015; 14(3):463-468 doi: 10.4314/tjpr.v14i3.15

© 2015 The authors.
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Abstract

Purpose: To evaluate the in vitro antioxidant and anti-neuroinflammatory activities of Hizikia fusiformis (Harvey) Okamura extract (HFE) in lipopolysaccharide (LPS)-stimulated murine BV-2 microglial cells.

Methods: The antioxidant activity of HFE was evaluated by measuring 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activity using an ESR spectrometer. Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators such as nitric oxide (NO), inducible NO synthase (iNOS) and Interleukin 6 (IL-6).

Results: Treatment with Hizikia fusiformis extract (HFE) significantly scavenged the DPPH radicals with half-maximal concentration of (IC₅₀) of 9.64 ± 0.78 μg/ml (p < 0.01 at 10 μg/ml). The increased levels of NO (35.32 ± 3.62 μM) and protein expressions of iNOS were inhibited by HFE extract in LPS-stimulated BV-2 cells. Increased pro-inflammatory cytokines such as TNF-α and IL-6, were also significantly suppressed by HFE (p < 0.001 at 80 μg/ml). Further, HFE blocked the expression of NF-κB activation in LPS-stimulated BV-2 cells.

Conclusion: HFE can be considered as a useful therapeutic and preventive approach for the treatment of neurodegenerative diseases and oxidative stress-related diseases.

Keywords: Hizikia fusiforme, Antioxidant activity, Anti-neuroinflammation activity, Inducible nitric oxide synthase, Interleukin-6