Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Structural identification of Lentinus edodes cellulose derivative that inhibits aflatoxin production by Aspergillus flavus

Hong-Bo Li¹ , Miao-Yan Wang², Liang-Bin Hu², Hai-Zhen Mo², Dao-Dong Pan¹

¹School of Marine Sciences, Ningbo University, Ningbo 315211; ²School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.

For correspondence:- Dao-Dong Pan Email: hongbo715@163.com Tel:+863737023380

Received: 27 September 2014 Accepted: 16 February 2015 Published: 31 March 2015

Citation: Li H, Wang M, Hu L, Mo H, Pan D. Structural identification of Lentinus edodes cellulose derivative that inhibits aflatoxin production by Aspergillus flavus. Trop J Pharm Res 2015; 14(3):495-501 doi: 10.4314/tjpr.v14i3.19

© 2015 The authors.
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Abstract

Purpose: To identify the structures of cellulose-extract derivative (CED) formed by heating Lentinus edodes cellulose in water surroundings that can efficiently inhibit aflatoxin production by Aspergillus flavus.

Methods: CED was purified on Sepharose CL-6B columns, and then structurally characterized using amino acid analyzer, gas chromatography, Fourier transform infrared spectroscopy, and polyacrylamide gel electrophoresis.

Results: CED completely inhibited aflatoxin AFB1 production by A. flavus at concentrations ≥ 100 µg/ mL. Chemical analysis indicated that CED contained 82 % carbohydrate and 18 % protein and has a molecular weight of approximately 24 kDa. Monosaccharide component analysis indicates that glucose was the predominant monosaccharide of CED. Analysis by Smith degradation and enzymatic hydrolysis indicate that there were only (1, 4)-glycosidic linkages in the CED polysaccharide chains. The protein backbone of CED contained 15 kinds of amino acid with higher levels of glutamic acid, aspartic acid, leucine and alanine.

Conclusion: CED was identified as a complex of peptide and polysaccharide structures possessing β-(1, 4)-glucan backbones, and it provides a theoretical basis for developing polysaccharide preparations to control aflatoxin contamination with medical and food science applications.

Keywords: Aflatoxin B1, Lentinus edodes, Aspergillus flavus, Cellulose derivative, Structure identification, Amino acid