Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antibacterial activity of the aqueous extract of Thonningia sanguinea against Extended-Spectrum-β-Lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae strains

JD N'guessan¹ , MR Dinzedi^1,2, N Guessennd², A Coulibaly¹, M Dosso^2,3, A J Djaman³, F Guede-Guina¹

¹Laboratoire de Pharmcodynamie Biochimique, 22 BP 582 Abidjan 22, Côte d'Ivoire; ²Département de Bactériologie & Virologie-Institut Pasteur de Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire; ³Laboratoire de Biochimie - Institut Pasteur de Côte d'Ivoire, 01 BP 490 Abidjan, Côte d'Ivoire.

For correspondence:- JD N'guessan Email: nguess100jd@yahoo.fr Tel:(00225) 05-78-57-89

Published: 16 September 2007

Citation: N'guessan J, Dinzedi M, Guessennd N, Coulibaly A, Dosso M, Djaman AJ, et al. Antibacterial activity of the aqueous extract of Thonningia sanguinea against Extended-Spectrum-β-Lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae strains. Trop J Pharm Res 2007; 6(3):779-783 doi: 10.4314/tjpr.v6i3.7

© 2007 The authors.
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Abstract

Purpose: The aim of this study was to evaluate the antimicrobial activity of Thonningia sanguinea against two sensitive and two multi-drug resistant (ESBL) Enterobacteria strains namely Escherichia coli and Klebsiella pneumoniae.

Method: The confirmation of the ESBL producing strains was done by the double-disc synergy tests and the broth dilution method was used for the determination of the antimicrobial parameters (MIC and MBC) on these sensitive and ESBL producing strains.

Results: The two sensitive strains had the same MIC and MBC values respectively 3.125 mg /ml and 12.50 mg/ml. The ESBL producing strains also had the same MIC of 6.25 mg /ml and MBC values of 25 mg/ml. The extract was bactericidal for all tested strains.

Conclusion: The results suggest that the flowers of T. sanguinea can be used in association with antibiotics for alternative therapy of diseases caused by ESBL producing E .coli, K. pneumoniae.

Keywords: antimicrobial activity, Thonningia sanguinea, ESBL producing strains; E .coli.