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Original Research Article | OPEN ACCESS

In vitro anticholinesterase and cholinergic effect of the aqueous extract of Trema guineensis on rabbit duodenum

Goueh Gnahoué1 , Jean David N'guessan1, Ernest Koffi2, Flavien Traoré3, Frédéric Guédé-Guina1

1Laboratoire de Pharmacodynamie Biochimique, UFR Biosciences Université d' Abidjan - Cocody, 22 BP 582 Abidjan 22; 2Laboratoire des sciences des Aliments, UFR Biosciences Université d' Abidjan - Cocody, 22 BP 582 Abidjan 22; 3Laboratoire de Physiologie Animale, UFR Biosciences Université d' Abidjan - Cocody, 22 BP 582 Abidjan 22, Côte d'Ivoire.

For correspondence:-  Goueh Gnahoué   Email: laurentgnahoue@yahoo.Fr   Tel:+22505801534

Received: 21 May 2008        Accepted: 25 October 2008        Published: 23 February 2009

Citation: Gnahoué G, N'guessan JD, Koffi E, Traoré F, Guédé-Guina F. In vitro anticholinesterase and cholinergic effect of the aqueous extract of Trema guineensis on rabbit duodenum. Trop J Pharm Res 2009; 8(1):11-17 doi: 10.4314/tjpr.v8i1.3

© 2009 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: In previous studies, the aqueous extract of Trema guineensis (Ulmaceae) was shown to increase rabbit duodenal contractions. The aim of this study was to evaluate the mechanism of the stimulation of the rabbit duodenum by the aqueous extract of Trema guineensis (Ulmaceae).
Methods: The in vitro organ bath method was used in our experiments. The enzyme was extracted from rabbit duodenum. The acetylcholinesterase (AChE) activity was determined by Ellman’s assay using acethylthiocholine iodide as substrate. The rate of hydrolysis of acetylcholinesterase was monitored at 412 nm using a spectrophotometer.
Results: The effective concentration which induces 50% effect of Hypo+ (EC50) was obtained with 8 x 10-2 mg/ml of Hypo+. The tests carried out in the presence of atropine showed a considerable reduction in the amplitudes of intestinal contractions. Hypo+ exerted mixed competitive inhibition on acetylcholinesterase (Vmax = 8.33 µM/min and KM= 6.25 x 10-4 M). These results indicate that the crude extract of Trema guineensis contains anti-AChE and cholinomimetic substances. These two properties can explain the increase of duodenal contraction by Hypo+.
Conclusion:These results support the use of Trema guineensis as a laxative due to its stimulating effects on duodenal contractility.

Keywords: Trema guineensis, Myostimulation, Acetylcholinesterase, Hypo+, Duodenal contraction

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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