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Original Research Article
Effect of Hawthorn on
Drosophila Melanogaster Antioxidant-Related Gene
Expression
Yan Zhang1,
Ting-ting Shen2, Su-wen Liu3,
Jiang Zhao1, Wen Chen1 and Hao
Wang1*
1Key Laboratory of
Food Nutrition and Safety, (Tianjin University of
Science and Technology), Ministry of Education,
2School of Biological
Engineering, Tianjin University of Science & Technology,
Tianjin 300457, 3Department of Food Science
and Technology, Hebei Normal University of Science and
Technology, Qin Huangdao, Hebei 066004, PR China
*For correspondence:
Email:
cpzyyan@163.com
Received: 4 Dece3mber 2013
Revised accepted: 13
February 2014
Tropical Journal of
Pharmaceutical Research, March 2014;
13(3):
353-357
http://dx.doi.org/10.4314/tjpr.v13i3.6
Abstract
Purpose:
To study the effects of
various doses of hawthorn extract on Drosophila
lifespan, antioxidant enzyme activity and expression of
antioxidant-related regulation genes.
Methods:
Experiments with Drosophila
as an animal model were conducted. The effects of
hawthorn on Drosophila melanogaster antioxidant related
gene expression were investigated by lifespan tests of
Drosophila, antioxidant enzyme activity assay of
Drosophila, and mRNA expression of antioxidant genes by
real time-PCR assay.
Results:
The results indicate that
hawthorn extract prolonged the life span of Drosophila,
with 50 % survival time of 0.8 and 4 mg/mL groups being
increased from 52 days (control) to 56 and 62 days,
respectively. Addition of 0.8 mg/mL hawthorn extract
increased CuZn-SOD enzyme activity significantly (p <
0.05); the 4 mg/mL extract
significantly increased CuZn-SOD enzyme (p < 0.01) and
CAT enzyme activity (p < 0.05), but decreased MDA
levels. Real time-PCR results show that the 4 mg/mL
extract significantly improved the expression levels of
CuZn-SOD and CAT mRNA (p < 0.05); on the other hand,
both extract concentrations improved PHGSH-Px mRNA level
significantly compared with that of control group (p <
0.05).
Conclusion:
The
antioxidant activity of hawthorn in vivo may be
achieved by increasing endogenous antioxidant enzymes.
Keywords:
Drosophila melanogaster,
Hawthorn, Lifespan, Enzyme, Gene expression,
Phospholipid hydroperoxide glutathione peroxidase,
Superoxide dismutase, Catalase |
