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Research Article
Cisplatin Induces Up-Regulation of KAI1, a
Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell
Line
Mohammad
Javad Mokhtari1*, Azim Akbarzadeh2,
Mehrdad Hashemi1, Gholamreza Javadi1,
Reza Mahdian3, Soheil Ghasemi2,
Ahmad Reza Kamiab1, Zhilla Hoseineian1,
Mohsen Chiani2 and Mahdieh Sadat Taghavi1
1Department of Biology, Science and
Research Branch, Islamic Azad University,
2Pilot
Biotechnology Department,
3Department of Molecular Medicine,
Biotechnology Research Center, Pasteur Institute of
Iran, Tehran, Iran.
*For correspondence:
Email:
mj_mokhtari@srbiau.ac.ir Tel:
+982144865154; Fax: +982144804181
Received:
14 October 2011
Revised accepted:
6
June 2012
Tropical
Journal of Pharmaceutical Research, August 2012;
11(4): 523-529
http://dx.doi.org/10.4314/tjpr.v11i4.1
Abstract
Purpose: To investigate the effect of
cisplatin on cell toxicity and metastasis through
modulation of KAI1 gene expression.
Methods: MCF-7cells
were incubated with different concentrations of
cisplatin for 24 h. RNA was extracted by trizol and cDNA
synthesized. KAI1 and TBP were chosen as target and
internal control genes, respectively. Specific primers
were designed by primer express software, v.3.0. KAI1/TBP
and gene expression ratio was calculated using the
formula, 2 -∆∆Ct.
Results: Cisplatin exerted a dose-dependent
inhibitory effect on the viability of highly metastatic
MCF-7 cells. KAI1/TBP gene expression ratios were 1.97 ±
0.19 (p < 0.05), 2.96 ± 0.55 (p < 0.05), 9.06 ± 0.27 (p
< 0.001) and 12.38 ± 0.88 (p < 0.01) in 10, 20, 50 and
100 μM concentrations of cisplatin.
Conclusion: These findings indicate that cisplatin can
inhibit metastasis by up-regulating KAI1 gene in
MCF-7cells.
Keywords: Cisplatin; KAI1; Metastasis; Breast
Cancer; Real-time PCR. |
