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Research Article
Antioxidant
Effect of Curcumin Against Microcystin-LR-Induced Renal
Oxidative Damage in Balb/c Mice
Saad
Al-Jassabi1,2, Khaled Abdul-Aziz Ahmed3,4*,
Mahmood Ameen Abdulla4
1Institute of Biological Sciences, Faculty of Science, University of
Malaya, 50603 Kuala Lumpur, Malaysia; 2Faculty
of Science, Yarmouk University, Irbid, Jordan; 3Department
of Medical Sciences, Faculty of Dentistry, Ibb
University, P.O.Box 70627, Ibb, Yemen; 4Department
of Molecular Medicine, Faculty of Medicine, University
of Malaya, 50603 Kuala Lumpur, Malaysia.
*For correspondence:
Email:
khaaah@gmail.com
Received:
14 July 2011 Revised
accepted: 1 June 2012
Tropical
Journal of Pharmaceutical Research, August 2012;
11(4): 5 31-536
http://dx.doi.org/10.4314/tjpr.v11i4.2
Abstract
Purpose: To investigate the effect of
curcumin on microcystin-LR (MC-LR)- induced renal
oxidative damage in Balb/c mice.
Methods: 40 male Balb/c mice were assigned
randomly to 4 groups each having 10 mice. One group
served as normal (saline treated) while another group
was used as curcumin control. The third group was given
MC-LR and used as toxin control. The fourth group was
pre-treated with curcumin (300 mg/kg body wt) given
orally once daily for 14 days before interperitoneal
injection (i.p) of MC-LR (75 µg/kg body wt). Biochemical
assays including serum creatinine, blood urea nitrogen
(BUN), urinary glucose, gamma-glutamyl transferase (GGT)
and catalase (CAT) levels were measured. Renal
biochemical tests such as protein carbonyl contents and
DNA-protein cross-links, glutathione peroxidase (GSH-Px)
activity, glutathione (GSH) and lipid hydroperoxide (LOOH)
were evaluated.
Results: Serum creatinine, BUN, urinary
glucose, GGT increased in mice treated with MC-LR, while
creatinine clearance decreased compared to controls (p <
0.001), indicating occurrence of tubular damage. There
was increased protein carbonyl content and DNA-protein
cross-links in the kidney homogenates of these mice.
Curcumin administration significantly reversed these
effects and attenuated the MC-LR-induced reduction in
the activities of CAT, GPH-Px, GSH as well as the MC-LR-induced
increases in plasma and kidney lipid hydroperoxide.
Conclusion: These results indicate that curcumin possesses
natural antioxidant properties that renders it a potent
protective agent against renal oxidative damage mediated
by microcystin-LR.
Keywords: Antioxidants, Curcumin, Microcystin,
Renal oxidative damage |
