http://dx.doi.org/10.4314/tjpr.v11i6.2
Abstract
Purpose: To evaluate Myt272 protein
antigenicity and immunogenicity by trial vaccination in
mice and its in silico analysis as a potential peptide
vaccine for tuberculosis.
Methods: Myt272 gene, which has 100 %
identity with Mycobacterium tuberculosis H37Rv unknown
function gene Rv3424c, was ligated by genomic shotgun
approach into the expression vector pQE32, and
transformed into Escherichia coli SG13009. Expression
during cell growth was induced by isopropyl-β-D-thiogalactopyranoside.
The recombinant protein was isolated from the harvested
cell lysate and injected in mice for immunogenicity
experiment up to 42 days. ELISA tests with anti-His
antibodies were performed on the collected individual
blood samples’ sera. Color development in a microplate
reader was measured at 450 nm.
Results: The protein was predicted to have a
mass of approximately 13 kDa and was present in the
soluble fraction of the cell lysate. The immunogenicity
test on Myt272 protein revealed very statistically
significant high levels of antibodies detected by ELISA
in the sera of immunized group of mice compared to
negative controls.
Conclusion: A 10.1 kD unnamed function (IDALA) protein from
Rv3424 gene could be the potential peptide vaccine for
tuberculosis tested by mice immunogenicity experiment.
Keywords: Tuberculosis H37Rv, Myt272 clone,
IDALA, Immunogenicity tests, In silico study.