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Research Article


Idala: An unnamed Function Peptide Vaccine for Tuberculosis

 

Salmah Ismail1, Nadiya Taha Darwish1, Teow Chong Teoh1*, Mahmood Ameen Abdulla2 and Rahmat Omar3

1Institute of Biological Sciences, Science Faculty, 2Department of Molecular Medicine, 3Department of Otorhinolaryngology, Medical Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia

*For correspondence: Email: : ttchong@um.edu.my  Tel: +603-79677189

Received: 10 May 2012                                     Revised accepted: 24 October 2012   

Tropical Journal of Pharmaceutical Research, December 2012; 11(6): 879-886

http://dx.doi.org/10.4314/tjpr.v11i6.2  

Abstract

 

Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice and its in silico analysis as a potential peptide vaccine for tuberculosis.

Methods: Myt272 gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was ligated by genomic shotgun approach into the expression vector pQE32, and transformed into Escherichia coli SG13009. Expression during cell growth was induced by isopropyl-β-D-thiogalactopyranoside. The recombinant protein was isolated from the harvested cell lysate and injected in mice for immunogenicity experiment up to 42 days. ELISA tests with anti-His antibodies were performed on the collected individual blood samples’ sera. Color development in a microplate reader was measured at 450 nm.

Results: The protein was predicted to have a mass of approximately 13 kDa and was present in the soluble fraction of the cell lysate. The immunogenicity test on Myt272 protein revealed very statistically significant high levels of antibodies detected by ELISA in the sera of immunized group of mice compared to negative controls.

Conclusion: A 10.1 kD unnamed function (IDALA) protein from Rv3424 gene could be the potential peptide vaccine for tuberculosis tested by mice immunogenicity experiment.

 

Keywords: Tuberculosis H37Rv, Myt272 clone, IDALA, Immunogenicity tests, In silico study.

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