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Original Research Article


Changes in the Characteristics of Water-in-Oil-based High Internal Phase Emulsion Containing Moringa Leaves Extract at Various Storage Conditions

 

Atif Ali1* and Naveed Akhtar2

1Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore, 2Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, the Islamia University of Bahawalpur 63100, Bahawalpur, Pakistan

 

*For correspondence: Email: ajmaline2000@gmail.com; Tel: +92 334 6081922; Fax: +92 62 9255243

 

Received: 6 April 2013                                                                            Revised accepted: 1 April 2014

Tropical Journal of Pharmaceutical Research, May 2014; 13(5): 677-682

http://dx.doi.org/10.4314/tjpr.v13i5.4   

Abstract

 

Purpose: To explore changes in the characteristics of water-in-oil-based high internal phase emulsion containing Moringa oleifera (Moringa HIPE) extract at various storage conditions and time intervals.

Methods: Moringa leaves extract (3 %) was entrapped into HIPE. Color, liquefaction, conductivity, pH, and centrifugation were assessed by keeping the samples of Moringa HIPE at 8, 25, 40 and 40 °C, and 75 % RH (relative humidity) over time up to 54 days. Rheological measurements were made on freshly prepared emulsion and after one and two months. Data were analysed using Brookfield Software Rheocalc version (2.6) with IPC Paste and Power Law (PL) math models.

Results: There was no change in color, electrical conductivity, liquefaction and phase separation (after centrifugation) in any sample of Moringa HIPE at the various storage conditions and periods. The pH of freshly prepared Moringa HIPE was 5.5 which gradually decreased with slight variation in the storage conditions. The viscosity of freshly prepared emulsion was 3256.65 cP and this decreased with increase in shear stress. Flow index and shear sensitivity factor of freshly prepared emulsion were 0.36 and 0.65, respectively. The rheograms of Moringa HIPEs indicate non-Newtonian behaviour and pseudo-plastic tendency. Power Law and IPC paste provided the data of confidences of fit.

Conclusion: Moringa HIPE showed stability and can be guided exclusively to protect skin against ultraviolet radiation-mediated oxidative damage.

 

Keywords: Moringa oleifera, High Internal Phase Emulsion, Rheogram, Pseudoplastic, Non-Newtonian, Shear

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