Tropical Journal of Pharmaceutical Research

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Research Article

Colorimetric Determination of Cefadroxil and Ceftriazone in Pharmaceutical Dosage Forms

Ch Aswani Kumar, BM Gurupadayya*, S Navya Sloka, RS Chandan and JC Thejaswini

Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS University, Mysore 570 015, (KA), India

*For correspondence: E-mail: bm_guru2004@yahoo.co.in Tel: +91-9242886136; Fax: +91-8212548359

Received: 5 July 2010 Revised accepted: 7 December 2010

Tropical Journal of Pharmaceutical Research, February 2011; 10(1): 81-88

Abstract

Purpose: To develop a simple, rapid and selective method for the spectrophotometric determination of cefadroxil and ceftriaxone using 1, 2- napthaquinone-4- sulfonic acid sodium.

Methods: The method was based on the derivatization of cefadroxil and ceftriaxone with 1, 2- naphthaquinone-4- sulfonic acid sodium in alkaline medium to yield orange-colored products.

Results: The reaction products of cefadroxil and ceftriaxone at their respective λ max of 475 and 480 nm showed linearity in the concentration range of 10 - 100 and 25 - 175 µg/ml, respectively. Relative standard deviations of 0.82 % for cefadroxil and 0.95 % for ceftriaxone were obtained. Recoveries of cefadroxil tablets and ceftriaxone injection were in the range of 100.66 ± 0.98 and 99.38 ± 0.84 %, respectively.

Conclusion: Recovery studies gave satisfactory results indicating that none of the major additives/excipients interfered with the assay method. Therefore, the proposed method is simple, rapid, precise and convenient for the assay of cefadroxil and ceftriaxone in commercial preparations.

Keywords: Cephalosporins, Derivatization, 1, 2- Naphthaquinone 4- sulfonic acid sodium, Colorimetry, Pharmaceutical formulation.