Absence of Mutagenicity in Three Nigerian Medicinal
Plants - Bidens pilosa, Cleistopholis paterns and
Tetrapleura tetraptera
Chang-Eui Hong1, Seang-Taek Ji2
and Su-Yun Lyu2*
1Dept.
of Pharmacy, Yeungnam University, Gyeongsan, 712-749,
2Dept. of Herbal Medicinal Pharmacology,
Daegu Haany University, 290 Yugok-dong Gyungsan,
Gyeonsangbuk-do 712-715, Korea
*
For
correspondence:
E-mail:
lyu@dhu.ac.kr
Tel: +82-53-819-1333, +82-10-3249-6083 (Mobile);
Fax: +82-53-819-1272
Received: 27 September
2010 Revised
accepted: 11 February 2011
Tropical
Journal of Pharmaceutical Research, April 2011; 10(2):
153-159
Abstract
Purpose: Bidens pilosa, Cleistopholis paterns,
and Tetrapleura tetraptera are plants that are used
traditionally for cancer treatment, as well as
anti-bacterial, and anti-inflammatory agents in Africa.
We used the Ames test to evaluate the bacterial reverse
mutation effects in these plants.
Methods: In the in vitro Ames test, Salmonella
typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and
Escherichia coli WP2uvrA were used with or without
metabolic activation by S9 mix. The highest
concentration of B. pilosa, C. paterns, and T.
tetraptera for the Ames test was established at 5000 μg/plate.
Results: B. pilosa, C. paterns, and T. tetraptera
did not cause any increase in the number of his+
revertant colonies compared to the negative control
values obtained from S. typhimurium and E. coli WP2uvrA
strains, with or without the addition of S9 mixtures.
The results indicated that these plants are
non-mutagenic to all the five S. typhimurium and E. coli
test strains in the presence and absence of metabolic
activation.
Conclusion: B. pilosa, C. paterns, and T.
tetraptera showed negative results in the bacterial
reverse mutation test, suggesting that it is potentially
safe to use them as medicinal plant supplements even at
high doses.
Keywords:
Bidens pilosa; Cleistopholis paterns; Tetrapleura
tetraptera; Ames test.
