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Research Article
Inhibition of
Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α
Expression by Aqueous Extract of Orixa
Japonica in RAW 264.7 Cells via Suppression of NF-κB
Activity
Chang-Hee Kang1, Yung Hyun Choi2,
Il-Whan Choi3, Jae-Dong Lee4 and
Gi-Young Kim1,*
1Laboratory
of Immunobiology, Department of Marine Life Sciences,
Jeju National University, Jeju 690-756;
2Department
of Biochemistry, College of Oriental Medicine, Dongeui
University, Busan 614-054;
3Department
of Microbiology, Inje University College of Medicine,
Busan 614-735, 4Department of Microbiology,
College of Natural Sciences, Pusan National University,
Busan 609-735, Republic of Korea
* For
correspondence:
E-mail:
immunkim@jejunu.ac.kr Tel:
+82-647543427
Received: 16 September
2010
Revised accepted: 19 January 2011
Tropical
Journal of Pharmaceutical Research, April 2011; 10(2):
161-168
Abstract
Purpose: To investigate the anti-inflammatory
effects of aqueous extract of Orixa japonica (AEOJ) in
lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Methods: The expression of mRNA and protein using
RT-PCR and Western blot analysis was investigated. The
level of nitric oxide (NO) production was analyzed using
Griess reaction. Release of prostaglandin E2
(PGE2) and tumor necrosis factor-α (TNF-α)
was determined using sandwich ELISA.
Results: AEOJ potently inhibited the production
of nitric oxide (NO), prostaglandin E2 (PGE2),
and tumor necrosis factor-α (TNF-α) in LPS-stimulated
RAW 264.7 cells. Consistent with these findings, AEOJ
was also found to significantly reduce LPS-induced
expression of inducible NO synthase (iNOS),
cyclooxygenase-2 (COX-2), and TNF-α at the
transcriptional level. Additionally, AEOJ attenuated
LPS-induced NF-κB activity via the inhibition of IκB
phosphorylation and degradation. It was also found that
the NF-κB inhibitor N-acetyl cysteine (NAC) attenuated
LPS-induced gene expression of iNOS, COX-2, and TNF-α.
These results indicate that AEOJ attenuates LPS-induced
inflammatory mediators such as NO, PGE2, and
TNF-α via suppression of NF-κB activity.
Conclusion: These results suggest that AEOJ has a
potential activity to alleviate LPS-induced
inflammation.
Keywords: Orixa japonica, Nitric oxide,
Prostaglandin E2, Tumor necrosis
factor-α, Nuclear factor-κB |
