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Research Article
Simultaneous
Quantification of Paracetamol and Meloxicam in Tablets
by High Performance Liquid Chromatography
Madhusudhanareddy Induri*, Bhagavan R Mantripragada2,
Rajendra P Yejella3, Pavankumar R Kunda2,
Meechel Arugula4 and Rajkumar Boddu1
*1Department
of Pharmaceutical Chemistry, Malla Reddy College of
Pharmacy, Maisammaguda, Dhulapally, Secunderabad, Andhra
Pradesh 500014, 2Department of Pharmaceutical
Chemistry, C.M. College of Pharmacy, Maisammaguda,
Dhulapally, Secunderabad, Andhra Pradesh 500014, 3Department
of Pharmaceutical Chemistry, College of Pharmaceutical
Sciences, Andhra University, Visakhapatnam, Andhra
Pradesh 530003, 4Department of Pharmaceutical
Analysis, St. Ann’s College of Pharmacy, Cantonment,
Vizianagaram, Andhra Pradesh 530003, India
For correspondence:
E-mail: msreddyinduri@yahoo.com;
Tel: +91-8125863579
Received: 5 December 2010
Revised
accepted: 26 June 2011
Tropical
Journal of Pharmaceutical Research, Aug 2011;
10(4): 475-481
http://dx.doi.org/10.4314/tjpr.v10i4.13
Abstract
Purpose:
To develop and validate a simple, rapid and inexpensive
RP-HPLC method for the simultaneous estimation of
paracetamol and meloxicam in tablets.
Methods:
For the analysis of the drugs, chromatographic analysis
was performed on XTerra symmetry C18 column
(100 × 4.6 mm, 5 µ particle size) with mobile phase
consisting of methanol and phosphate buffer (pH 9.2) in
the ratio of 50:50 v/v, at a flow rate of 0.8 mL/min and
eluents monitored at 244 nm. The method was validated
for linearity, accuracy, precision, robustness and
application for assay as per International Conference on
Harmonization (ICH) guidelines.
Results:
The retention times of paracetamol and meloxicam were
2.467 and 4.971 min, respectively. The calibration
curves of peak area versus concentration, which was
linear from 5 - 60 µg/mL for paracetamol and 1 - 12 µg/mL
for meloxicam, had regression coefficient (r2)
greater than 0.999. The method had the requisite
accuracy, precision, and robustness for simultaneous
determination of paracetamol and meloxicam in tablets.
Conclusion:
The proposed method is simple, low-cost, accurate,
precise and can be successfully employed in routine
quality control for the simultaneous analysis of
paracetamol and meloxicam in tablets.
Keywords:
Paracetamol, Meloxicam, RP-HPLC, Simultaneous analysis,
Tablets. |
