Photoprotective Effects of Hydroalcohol Tagetes
Erectus Extract
Against UV-Induced Oxidative Damage in Mice
Monika
Sachdeva1*,
Murli Dhar Kharya2,
Ahmed Aljarbou3 and Taruna Katyal4
1College
of Pharmacy, Qassim University, Buraidah, Saudi Arabia ,2Department
of Pharmaceutical Sciences, Dr. H.S Gour University,
Sagar, M.P., India, 3College of
Pharmacy, Qassim University, Buraidah, Saudi Arabia,
4I.S.F. College of Pharmacy, Moga, Panjab,
India.
For correspondence:
E-mail:
monikasachdeva@rediffmail.com Tel:
0096663802446 Ext. 6220
Received: 13 January
2011 Revised
accepted: 24 November, 2011
Tropical
Journal of Pharmaceutical Research, December 2011;
10(6): 747-753
http://dx.doi.org/10.4314/tjpr.v10i6.7
Abstract
Purpose:
To investigate the effects of topical application of
Tagetes erectus hydroalcohol extract as a dermal
antioxidant agent and evaluate its capacity to prevent
ultraviolet (UV) –induced oxidative damage.
Methods:
The plant flower was extracted with aqueous ethanol (60
%). Female Lacca mice were divided into five groups of
24 animals each. Group I was un-irradiated control
(neither UV exposure nor any treatment received). Group
II was irradiated control and received 5 min UV exposure
twice a day. Groups III, 1V and V received both UV
exposure and treatment of different concentrations of
the extract, 4 h. prior to UV exposure. The degree of
protection was quantified using biochemical tests (lipid
peroxidation and glutathione level) and
histopathological assessment.
Results:
The results showed that 2% topical extract treatment
reduced the effect of UV light-induced photoaging on
mice skin by decreasing malondialdehyde (MDA) level by
up to 50 % and increasing glutathione (GSH) level
3-fold (p < 0.01) compared to UV-irradiated control
group. Histopathological evaluation also indicated a
photo-protective effect on the extract-treated mice skin
as no signs of histological changes were seen after UV
exposure.
Conclusion:
Topical application of T .erectus has a potential for
preventing oxidative damage by UV irradiation.
Keywords:
T.erectus , Photo-ageing,
skin wrinkles, Antioxidants, Oxidative damage, UV
radiation
.
