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Research Article
A
Protease Isolated from the Latex of Plumeria rubra
Linn (Apocynaceae) 2: Anti-inflammatory and
Wound-Healing Activities
Indranil Chanda1*,
Usha Sarma2, Sanat K Basu3,
Mangala Lahkar4 and Sadhan K Dutta5
1Girijananda
Chowdhury Institute of Pharmaceutical Science, Guwahati,
Assam-781017, 2Department of Pathology,
Gauhati Medical College and Hospital, Guwahati,
Assam-781032, 3Department of Pharmaceutical
Technology, Jadavpur University, Kolkata, West
Bengal-700032, 4Department of Pharmacology,
Gauhati Medical College and Hospital, Guwahati,
Assam-781032, 5A College of Pharmacy, Bengal
School of Technology, Hooghly, West Bengal- 712102,
India.
For correspondence:
E-mail:
ichanda@sify.com Tel: +919957179226
Received: 12 May
2011 Revised
accepted: 15 October, 2011
Tropical
Journal of Pharmaceutical Research, December 2011;
10(6): 755-760
http://dx.doi.org/10.4314/tjpr.v10i6.8
Abstract
Purpose:
The aim of this
study was to investigate the anti-inflammatory and
wound-healing activities of the protease isolated from
the latex of Plumeria rubra Linn.
Methods:
The protease was isolated from the latex of the plant by
acetone precipitation method and given a trivial name,
Plumerin-R. The anti-inflammatory activity of the
protease was based on its effects on carrageenan-induced
paw oedema in rats. Its wound healing effect was
investigated using an excision wound rat model.
Results:
Four hours after
treatment, the reduction in carrageenan-induced rat paw
oedema by 20, 40 and 80 mg/kg body weight of plumerin-R
was 21.6, 33.8 and 48.8 %, respectively, while oedema
reduction by indomethacin (10 mg/kg) was 58.0 %.
Plumerin-R showed significant (p < 0.05) wound closure
and epithelialization time compared with control.
Conclusion:
This study
demonstrates that the protease, Plumerin-R, has
significant anti-inflammatory and wound-healing
properties.
Keywords:
Protease, Plumerin-R, Anti-inflammatory, Excision wound,
Healing. |
