University Department of Pharmaceutical Sciences,
Utkal University, VaniVihar Bhubaneswar, Orissa, India
*For correspondence:
Email:
amulya_slmp@rediffmail.com
Received: 23 July
2011 Revised
accepted: 16 March 2012
Tropical
Journal of Pharmaceutical Research, June 2012;
11(3): 345-350
http://dx.doi.org/10.4314/tjpr.v11i3.2
Abstract
Purpose:
To
formulate and evaluate glibenclamide (GB)-loaded
poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs)
for controlled release.
Methods: GB-loaded PLGA NPs were prepared by
solvent evaporation technique using
methanol/dichloromethane (2:1) and characterized by transmission
electron microscopy (TEM), and differential scanning
calorimetry (DSC). The effect of stirring speed (250,
1000, 1500 and 2500 rpm) and drug: polymer ratio (1:1,
1: 2, 1:3 and 2:1) on particle size, size distribution,
zeta potential, drug loading, encapsulation efficiency
and drug release was also studied.
Results:
Stable NPs were successfully prepared without any
incompatibility, as indicated by TEM and DSC studies,
respectively. As polymer and drug concentrations, and
stirring speed increased, particle size, drug loading
and encapsulation efficiency also increased. Increase in
polymer concentration sustained drug release but reverse
was obtained as drug concentration increased.
Conclusion: Controlled release biodegradable glibenclamide
NPs can be efficiently prepared by emulsification
solvent evaporation method suitably modulating
processing variables.
Keywords:
Poly (lactic-co-glycolic) acid, Nanoparticles,
Glibenclamide, Transmission electron microscopy,
Differential scanning calorimetry
