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Research Article
Effect of Bridelia ferruginea (Euphorbiaceae)
Leaf Extract on Sucrose-induced Glucose Intolerance in
Rats
Dieudonne
Njamen1, Benedicta N Nkeh-Chungag2,
Emmanuel Tsala3, Zacharias T Fomum 3,
Jean Claude Mbanya4 and George F Ngufor5
1
Department of Animal Biology
and Physiology, Faculty of Science, University of
Yaounde1, PO Box 812, Yaoundé, Cameroon, 2
Department of Zoology, Faculty of Science, Engineering
and Technology, Walter Sisulu University, Mthatha, 5117,
South Africa, 3Department of Organic
Chemistry, Faculty of Science, University of Yaoundé 1,
PO Box 812 Yaoundé, 4Department of Internal
Medicine, Faculty of Medicine and Biomedical Sciences,
University of Yaounde1, 5Department of
Health, Yaoundé, Cameroon.*For correspondence:
Email:
bnkehchungag@wsu.ac.za Tel/Fax: (+27) 47 502 1989
Received: 10
March
2012
Revised accepted: 12 September 2012
Tropical
Journal of Pharmaceutical Research, October 2012;
11(5): 759-765
http://dx.doi.org/10.4314/tjpr.v11i5.9
Abstract
Purpose: To evaluate the hypoglycaemic effect
of the methanol extract of Bridelia ferruginea leaves (MEBF)
on sucrose-induced glucose intolerance in rats.
Methods: Male Wistar rats, aged 6 - 7 weeks
and weighing 140 - 160 g, were used. The animals were
fed standard rat chow supplemented with 35%, 50% or 65%
sucrose for 8 weeks while control animals were fed
standard rat chow. The hypoglycaemic effect of MEBF and
the reference drugs (tolbutamide, and metformin) in the
animals were evaluated following a single dose of these
drugs and 6-day treatment. Plasma lipid profiles were
also determined.
Results: Fasting glucose concentrations
ranged from 45 to 70 mg/dl, and the increase was
significant in the sucrose diet groups from week 1.
After 2 weeks on these diets, oral glucose tolerance
test showed that sucrose feeding significantly impaired
glucose homeostasis 1 and 2 hours after a glucose
challenge (76.7 ± 2.0 versus 86.4 ± 8.5 and 66.7 ± 1.4
versus 75.5 ± 3.0, respectively). Fasting blood sugar
levels were significantly (p < 0.05) reduced in
sucrose-induced, glucose-intolerant rats after a single
dose of MEBF. The extract also significantly reduced
blood glucose (from 167 ± 23 mg/dL to 126 ± 5 mg/dL),
serum total cholesterol (from 161 ± 20 mg/dL to 93 ± 10
mg/dL) and triglyceride levels (281 ± 25 mg/dL to 228 ±
5 mg/dL) in glucose intolerant rats after 6 days of
treatment.
Conclusion: The methanol leaf extract of
Bridelia ferruginea exhibited hypoglycaemic effect in
glucose-intolerant rats.
Keywords: Sucrose-induced, Glucose
intolerance, Bridelia ferruginea, Hypoglycaemia,
Metformin, Tolbutamide. |
