http://dx.doi.org/10.4314/tjpr.v11i6.12
Abstract
Purpose: This study aimed to evaluate the
effects of topically applied water-in-oil (w/o) emulsion
of Hippophae rhamnoides using standard R cutometer
parameters.
Methods: A w/o emulsion of 1 %
hydro-alcoholic extract of H. rhamnoides (formulation)
and placebo control (base) were used in the study.
Eleven healthy male volunteers with a mean age of 24.5
years were selected after obtaining informed consent.
The subjects were assigned to blindly use either the
formulation or the base for 7 consecutive weeks. The
skin mechanical parameters determined with a cutometer,
were R0 (first maximum amplitude), R2
(gross-elasticity), R6 (viscoelasticity), R7 (biological
elasticity) and R8 (total recovery). In addition, the
antioxidant activity of the formulation was evaluated by
1, 1-diphenil-2-picrylhydrazyl (DPPH) method.
Results: Topical application of 1 % organic
extract emulsion of H. rhamnoides improved most of the
biomechanical parameters evaluated when compared to the
base group (p < 0.05). However, skin extensibility and
firmness of the active formulation- and base-treated
groups (R0) were not different (p < 0.01). Of note, a
significant correlation between the active formulation
and the improvement of the skin mechanical parameters
was observed. The active formulation was found to be
superior than the placebo control.
Conclusion: The topical antioxidant emulsion of
H. rhamnoides significantly improved skin biomechanical
parameters after 7 weeks of treatment. The data obtained
suggest that H. rhamnoides could be an alternative
pharmacological tool for treating age-related loss of
skin elasticity.
Keywords:
Skin elasticity, Skin aging, Emulsion, Cutometer,
Hippophae rhamnoides