Determination of
Venlafaxine and Modafinil in Individual Tablet Dosage
Forms using Single RP-HPLC Method
(RETRACTED)
Mohammad Younus1*,
Md Fasiuddin Arif2, M Paul Richards3
and D Bharat Kumar4
1Chandra Labs, 2Dr.
Reddy’s Laboratories Ltd, 3Malla Reddy
College of Pharmacy, Hyderabad Andhra Pradesh India,
4Vignan Institute of Pharmaceutical Sciences,
Nalgonda Dist, Andhra Pradesh, India
*For
correspondence: E-mail:
mdyounus1127@gmail.com Tel:
+91-8686062421
Received: 19 June
2012
Revised accepted:
30 January 2013
Tropical
Journal of Pharmaceutical Research, April 2013;
12(2): 239-245
http://dx.doi.org/10.4314/tjpr.v12i2.17
Abstract
(RETRACTED)
Purpose:
To
develop a simple and selective isocratic method for the
determination of venlafaxine and modafinil in tablet
dosage forms.
Methods:
The compounds were analyzed on Waters symmetry C18
column (4.6 mm x 250 mm i.d, 5µm) using a mobile phase
consisting of a mixture of ammonium acetate buffer (pH
was adjusted to 4.0 with glacial acetic acid):10 %
methanol in acetonitrile, in the ratio of 60:40. The
flow rate was 1.0 ml/min and column effluents were
monitored at 225 nm. The method was validated according
to ICH guidelines.
Results:
Venlafaxine and modafinil were eluted with retention
times of 4.416 min and 6.443 min, respectively. The
method was linear in the range of 1.0 - 50 µg/ml for
both venlafaxine and modafinil. The relative standard
deviation (%RSD) was < 1 for both drugs while mean
recovery values at different concentration levels were
within limits. The performance of the method was not
changed when small variations in the method were made.
Conclusion:
The proposed method is accurate, reproducible and
low-cost, and can be used for the routine analysis of
the individual drugs in formulations.
Keywords: Venlafaxine, Modafinil, Isocratic
method, Validation
