Molecular Characterization of
Glucose-6-Phosphate
Dehydrogenase
Deficiency in a University Community in Malaysia
Ahmed M Sulaiman1, Sultan AM Saghir2*,
Faisal M Al-Hassan1, Narazah M
Yusoff1 and
Abdel-Hamid A Zaki1
1Advanced
Medical and Dental Institute (AMDI), Universiti Sains
Malaysia (USM), No 1-8 (Lot 8), Persiaran
Seksyen 4/1, Bandar Putra Bertam, 13200 Kepala Batas. Pulau
Pinang, 2School
of Pharmaceutical Sciences, Pharmacology Department,
Penang, Malaysia
*For correspondence:
Email:
sultan_a1976@yahoo.com Tel:
0060142415953
Received: 23 August 2012
Revised accepted:
7 May 2013
Tropical Journal of
Pharmaceutical Research, June 2013;
12(3):
363-367
http://dx.doi.org/10.4314/tjpr.v12i3.14
Abstract
Purpose:
To determine the
prevalence of glucose-6-phosphate
dehydrogenase (G6PD) deficiency among staff
and students of a university community in
Malaysia as well as to identify molecular
genetics by determination of G6PD mutations.
Methods: Cross-sectional and
experimental studies were carried out on the
staff and students of Advanced Medical and
Dental Institute (AMDI) of Universiti Sains
Malaysia (USM) from July 2009 to April 2010.
Venous blood samples were collected from 87
individuals (45 males and 42 females), all of whom gave
informed consent. Full blood count,
reticulocyte count, screening test and
quantitative determination of G6PD were
performed. The deficient subjects were
confirmed by standard PCR restriction
enzyme. DNA samples from these subjects were
analyzed for certain known G6PD mutations by
digesting them with appropriate restriction
enzymes.
Results:
Out of the 87 subjects (80 were Malay, 2
were Chinese, 1 was Indian and 4 were
others). The total prevalence of
G6PD deficiency among the subjects was 4.59
% (4/87), all of whom were Malay males.
One of the deficient
subjects had G6PD Viangchan, while the other
three were G6PD Mahidol (487 G>A).
Conclusion: The finding of this
study demonstrate that the most common
mutation among AMDI staff and students is
Mahidol (487G>A), followed by mutation
Viangchan (871G>A).
Keywords:
Glucose-6-Phosphate Dehydrogenase, Mahidol,
Viangchan, Haemolytic anaemia
