Acylated Flavonoid from
Vaccinium Corymbosum (Ericaceae) Flowers with Yeast
α-Glucosidase Inhibitory Activity
Chunpeng Wan1*
and Shouran Zhou2
1Jiangxi Key Laboratory for
Postharvest Technology and
Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi
Agricultural University, Nanchang 330045,
2Jiangxi University of
Traditional Chinese Medicine, Nanchang 330006, China
*For correspondence:
lemonwan@126.com
Tel: +86 791 83813185; Fax:
+86 791 83813185
Received: 21 September 2012
Revised accepted:5 June
2013
Tropical Journal of
Pharmaceutical Research, August 2013; 12(4): 549-552
http://dx.doi.org/10.4314/tjpr.v12i4.16
Abstract
Purpose:
To isolate and identify
chemical constituents with yeast α-glucosidase
inhibitory activity from the methanol extract of
V. myrtillus (blueberry) flowers.
Methods:
The active fraction (ethyl
acetate extract) were chromatographed on
C18 MPLC column, Sephadex LH-20 column and
semi-preparative HPLC column. The isolated compounds
were identified by the extensive 1H-nuclear
magnetic resonance spectroscopy (NMR), 13C-NMR,
2D-NMR and high resolution mass spectral (HR-MS)
analyses.
Results:
Two phenolic compounds, an
acylated flavonoid and a coumaric acid derivative, were
isolated and identified as isorhamnetin-3-O-(6''-O-coumaroyl)-β-D-glucoside
(1) and cis
-cinnamic acid methyl
(2). Compound 1 showed
powerful α-glucosidase inhibitory activity and in this
regard, was superior to the positive drug, acarbose.
Conclusion:
Compounds 1
and 2 were
isolated for the first time from this species and the
genus of Vaccinium. This is the
first report on characterization of these phenolic
compounds and the possible utilization of blueberry
flowers for nutraceutical and functional food
applications.
Keywords:
Vaccinium corymbosum, Blueberry, Acylated flavonoid,
Yeast
α-Glucosidase, Inhibitory
activity, Nutraceuticals
