High Performance Liquid
Chromatographic Determination of Phenolic Compounds in
Propolis
Li Yang1, 3,
Qing-Hua Yan2, Jin-You Ma1,
Qing Wang1, Jian-Wei Zhang1
and Guo-Xi Xi3,*
1Department of Experimental
Center, Henan Institute of Science and Technology,
2Department of Life Science
and Technology, Xinxiang Medical University, Xinxiang,
Henan 453003, 3School of Environment, Henan
Normal University. Key Laboratory for Yellow River and
Huai River Water Environment and Pollution
Control，Ministry of Education. Henan Key Laboratory for
Environmental Pollution Control, Xinxiang, Henan 453007,
PR China.
*For correspondence:
Email:
syzxhome@163.com; Tel:
+86-373-3040746
Received: 6 November 2012
Revised accepted: 10 June 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
771-776
http://dx.doi.org/10.4314/tjpr.v12i5.17
Abstract
Purpose:
To develop a simple,
precise and rapid high-performance liquid
chromatographic technique coupled with photodiode
array detection (DAD) method for the simultaneous
determination of rutin, quercetin, luteolin,
genistein, galangin and curcumin in propolis.
Methods: Ultrasound-assisted
extraction was applied to isolate six antioxidant
phenolic compounds from propolis samples.
Chromatographic separation was performed using an
Eclipse XDB-C18 column (150 mm×4.6 mm, 5 μm) by
isocratic elution at 25 °C. The mobile phase
consisted of phosphate buffered saline (pH = 4.5)
and methanol (40/60, v/v). The flow rate was set at
0.8 ml/min and the effluent was monitored using a
DAD set at 260 nm.
Results: Excellent linear
correlation coefficient was observed in the range of
0.9977 to 0.9997. The reproducibility of the method
was 1.58 % (RSD) and accuracy was confirmed with
average recovery ranging from 95.71 to 104.26 %. Six
antioxidant phenolic compounds in propolis samples
were successfully determined within 50 min.
Conclusion: The proposed isocratic LC
method gave good separation and accurate
determination of rutin, quercetin, luteolin,
genistein, galangin and curcumin in propolis. The
proposed method showed good sensitivity, linearity,
repeatability and accuracy.
Keywords:
Propolis, Rutin, Quercetin, Luteolin, Genistein,
Galangin, Curcumin, HPLC.
