School of Pharmaceutical
Sciences, Universiti Sains Malaysia, Penang, Malaysia
*For correspondence:
Email:
layjing80@yahoo.com;
Tel: +604-6533888 ext 4711;
Fax: +604-6570017
Received: 12 August 2012
Revised accepted: 12 July 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
777-782
http://dx.doi.org/10.4314/tjpr.v12i5.18
Abstract
Purpose: To carry out
phytochemical and physicochemical studies on the
leaves of Gynura segetum.
Methods: Preliminary phytochemical
and physicochemical studies using chromatographic
techniques,were carried out, Parameters evaluated
include ash value, loss on drying and extractive value,
amongst others. .
Results: Phytochemical results
revealed that the leaves of Gynura segetum contain
alkaloid, terpene, flavonoid, tannin and saponin. Mean
ash value (%) was 13.5 (total), 2.49 (acid-insoluble
ash) and 6.14 (water-soluble ash). Loss on drying was
10.70 % while extractive value was 20.60 % in water and
14.13 % in ethanol. Thin layer chromatography (TLC)
analysis indicates the presence of rutin in the methanol
extract of G. segetum and this was supported by high
performance liquid chromatography (HPLC) studies.
Conclusion: Useful quantitative and
descriptive data essential for identifying and
characterizing the plant for the purpose of quality
control are presented.
Keywords: Gynura segetum, Quality
control, Extractive, Ash value
.
