Adsorptive Cathodic
Stripping Voltammetric Determination of Ciprofloxacin in
Bulk Powder, Pharmaceutical Dosage Forms and Urine
Vu Dang Hoang*
and Nguyen Thi Yen
Department of Analytical
Chemistry and Toxicology, Hanoi University of Pharmacy,
13-15 Le Thanh Tong, Hanoi, Vietnam
*For correspondence:
Email:
hoangvd@hup.edu.vn;
Tel: +84-438-254539; Fax:
+84-438-264464
Received: 18 October
2012
Revised accepted:
12 June 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
783-7
90
http://dx.doi.org/10.4314/tjpr.v12i5.19
Abstract
Purpose: To investigate the
electro-reduction behaviour and determination of
ciprofloxacin using a hanging mercury drop electrode.
Methods: Cyclic voltammograms of
ciprofloxacin recorded in Britton – Robinson buffers pH
2 – 5 exhibit a single irreversible cathodic peak. The
dependence of the peak current and peak potential values
on buffer (nature, pH and concentration) and
accumulation conditions (time and potential) were
examined and used for the quantitative analysis of
ciprofloxacin in dosage forms and urine. Acetate buffer
(0.08 M, pH 3.6) was selected as a supporting
electrolyte for quantitative purposes by differential
pulse and square wave adsorptive cathodic stripping
voltammetry.
Results: A reduction wave was seen
in the range of – 1.3 to – 1.5 V. These techniques were
successfully validated as per ICH guidelines and
comparable to US Pharmacopoeia HPLC method. The
techniques was applied to the determination of
ciprofloxacin in pharmaceutical formulations (i.e.
precision (RSD < 1%) and accuracy (99 – 101%)), and was
further extended to determine ciprofloxacin in spiked
human urine with no matrix effect (i.e. LLOQ 0.01 mg/ml,
precision (RSD < 15%) and accuracy (85 – 115%)).
Conclusion: Validated adsorptive
cathodic stripping voltammetric technique can be
recommended for use in quality control and
pharmacokinetics studies.
Keywords: Ciprofloxacin, Mercury
electrode, Adsorptive cathodic stripping voltammetry,
Pharmaceutical formulation, Urine.
