Production of Methioninase
from Serratia Marcescens Isolated from Soil and
its Anti-Cancer Activity against Dalton’s Lymphoma
Ascitic (DLA) and Ehrlich Ascitic Carcinoma (EAC) in
Swiss Albino Mice
William Arputha Sundar1*
and Hariharan Nellaiah2
1Sankaralingam Bhuvaneswari
College of Pharmacy, Anaikuttam, Sivakasi – 626130,
2Department of Biotechnology, Kalasalingam
University, Anand Nagar, Krishnankoil – 626126,
Tamilnadu, India.
*For correspondence:
Email:
williamas@rediffmail.com;
Tel: +91-9600338289
Received:
25 June 2013
Revised accepted:
25 June 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5): 699-704
http://dx.doi.org/10.4314/tjpr.v12i5.6
Abstract
Purpose: To evaluate the anticancer activity
of an enzyme, methioninase, obtained from a soil
isolate, Serratia marcesens, against Dalton’s lymphoma
ascitic (DLA) and Ehrlich ascitic carcinoma (EAC) in
Swiss albino mice.
Methods: Cancer was induced using DLA and EAC
cells in Swiss albino mice by intraperitoneal injection.
The mice were then treated with 10 and 20 mg/kg body
weight of methioninase; 5-fluorouracil (5-FU) was used
as standard.
Results: The results showed good improvement
in haematological parameters, improvement serum enzymes
and lipid profile, as well as increase in life span by
up to 77 %, increase in body weight (p < 0.01), decrease
in cancer cell count from (2.70 ± 0.40) × 106
to (1.86 ± 0.30) × 106 cells/ml compared to
(2.70 ± 0.40) × 106 to (1.42 ± 0.25) x 106
cells/ml for the group treated with 5-FU. Solid tumor
volume decreased from 5.82 ± 0.15 to 4.10 ± 0.11 ml as
as against a decrease from 5.82 ± 0.40 to 3.56 ± 0.28
for the group treated with 5-FU.
Conclusion: The isolated enzyme, methioninase,
has a good anti-cancer activity and needs to be further
be investigated for this activity.
Keywords: Methioninase, Anticancer, Serum
depletion, Tumor, Dalton’s lymphoma ascetic, Ehrlich
ascitic carcinoma
